| 邻苯二甲酸二(2-乙基)己酯诱导大鼠尿道下裂及对阴茎TGF-β1、TGF-βR3表达的影响 |
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| 引用本文： | 陈柏林,刘星,吴盛德,袁心刚,何昀,徐明灯,刘东尧,温晟,魏光辉. 邻苯二甲酸二(2-乙基)己酯诱导大鼠尿道下裂及对阴茎TGF-β1、TGF-βR3表达的影响[J]. 生殖与避孕, 2011, 31(2): 88-92,101 |
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| 作者姓名： | 陈柏林 刘星 吴盛德 袁心刚 何昀 徐明灯 刘东尧 温晟 魏光辉 |
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| 作者单位： | 重庆医科大学附属儿童医院泌尿外科,儿童发育疾病研究省部共建教育部重点实验室,重庆,400014 |
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| 基金项目： | 科技部十一五科技支撑计划(No.2006BAI05A06); 高等学校博士学科点专项科研基金(No.200806310005)项目 |
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| 摘 要： | 目的:探讨诱导建立大鼠先天性尿道下裂,研究邻苯二甲酸二(2-乙基)己酯(DEHP)及胎鼠阴茎内转化生长因子(TGF-β1,TGF-βR3)在尿道下裂发生机制中的意义。方法:SD孕鼠随机分为DEHP组(每日500 mg/kg)和大豆油对照组,每组20只。分别于母鼠孕12 d至19 d(GDl2～19)持续经口灌注给药。每组分别留取10只孕鼠让其正常分娩,出生第1日计数新生大鼠,并在解剖显微镜下测量雄性新生鼠的肛门生殖器距离(AGD)和称体质量;雄性仔鼠70日龄时逐个检查尿道下裂的发生情况。另10只孕鼠于孕第19日行剖宫产取仔鼠,应用逆转录-聚合酶链反应(RT-PCR)的方法检测胎鼠阴茎TGF-β1、TGF-βR3 mRNA的表达水平。结果:DEHP成功诱导出尿道下裂大鼠动物模型,DEHP组仔鼠体质量及AGD较对照组明显缩小(P<0.05)。DEHP组胎鼠阴茎TGF-β1、TGF-βR3 mRNA表达水平增加(P<0.05)。结论:DEHP成功诱导建立先天性尿道下裂大鼠模型,胎鼠阴茎内TGF-β1、TGF-βR3异常表达可能是尿道下裂的发生机制之一。
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| 关 键 词： | 邻苯二甲酸二(2-乙基)己酯 尿道下裂 TGF-β1 TGF-βR3 |
Di(2-ethylhexyl)phthalate Induces Hypospadias in Rat and Its Effecton the Expression of TGF-b1,TGF-bR3 in Genital Tubercles |
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| Bai-lin CHEN,Xing LIU,Sheng-de WU,Xing-gang YUAN,Yun HE,Ming-deng XU,Dong-yao LIU,Sheng WEN,Guang-hui WEI. Di(2-ethylhexyl)phthalate Induces Hypospadias in Rat and Its Effecton the Expression of TGF-b1,TGF-bR3 in Genital Tubercles[J]. Reproduction and Contraception, 2011, 31(2): 88-92,101 |
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| Authors: | Bai-lin CHEN Xing LIU Sheng-de WU Xing-gang YUAN Yun HE Ming-deng XU Dong-yao LIU Sheng WEN Guang-hui WEI |
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| Affiliation: | Bai-lin CHEN,Xing LIU,Sheng-de WU,Xing-gang YUAN,Yun HE,Ming-deng XU,Dong-yao LIU,Sheng WEN,Guang-hui WEI(Department of Pediatric Urology Surgery,Children's Hospital of Chongqing Medical University,Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing,400014) |
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| Abstract: | Objective:To induce hypospadias in rat with di(2-ethylhexyl) phthalate(DEHP),andinvestigate the expression of TGF-b1,TGF-bR3 in fetal rat genital tubercles.Methods:Pregnant SD rats wererandomly divided into 2 groups with 20 in each group:DEHP(500 mg/kg) group and the control.Rats weregavaged from gestation day 12 to 19(GDl2-19).Ten pregnant rats were let to delivery normally in each group.After the pregnant rats had delivered,the male offsprings were counted and the anal genital distance(AGD)and body weight... |
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| Keywords: | di(2-ethylhexyl) phthalate(DEHP) hypospadias TGF-b1 TGF-bR3 |
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