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| 瑞芬太尼对大鼠脑缺血再灌注损伤后大脑皮质bcl-2和Caspase-3表达的影响 | |
| 引用本文： | 舒榕,刘冬玲,王强. 瑞芬太尼对大鼠脑缺血再灌注损伤后大脑皮质bcl-2和Caspase-3表达的影响[J]. 华中科技大学学报(医学版), 2012, 41(1): 72-75. DOI: 10.3870/j.issn.1672-0741.2012.01.016 |
| 作者姓名： | 舒榕 刘冬玲 王强 |
| 作者单位： | 1. 湖北省中山医院麻醉科,武汉,430033 2. 武汉市普爱医院病理科,武汉,430030 3. 武汉科技大学医学院基础医学部免疫学教研室,武汉,430086 |
| 基金项目： | 湖北省自然科学基金资助项目 |
| 摘 要： | 目的 研究瑞芬太尼对大鼠脑缺血再灌注损伤后bcl-2和Caspase-3表达的影响,探讨瑞芬太尼对脑缺血再灌注损伤的作用.方法 以线栓法制作大鼠大脑中动脉阻塞的脑缺血再灌注模型,采用免疫组化、Real-time PCR法和神经行为检测相结合的.方法,观察缺血再灌注侧大脑皮质内bcl-2和Caspase-3的表达及神经功能的变化.结果 与缺血再灌注组比较,瑞芬太尼组的bcl-2 mRNA实时表达明显增高(P＜0.05),Caspase-3 mRNA实时表达明显降低(P＜0.05),神经异常行为明显减轻.结论瑞芬太尼可上调大鼠脑缺血再灌注损伤后bcl-2的表达,下调Caspase-3表达,明显改善神经症状,对脑缺血再灌注损伤有神经保护作用. |
| 关 键 词： | 瑞芬太尼 bcl-2 Caspase-3 |
Effect of Remifentanil on Expression of Bcl-2 and Caspase-3 in RatsCerebral Cortex Following Ischemia-reperfusion Injury |
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| Shu Rong,Liu Dongling,Wang Qiang. Effect of Remifentanil on Expression of Bcl-2 and Caspase-3 in RatsCerebral Cortex Following Ischemia-reperfusion Injury[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2012, 41(1): 72-75. DOI: 10.3870/j.issn.1672-0741.2012.01.016 | |
| Authors: | Shu Rong Liu Dongling Wang Qiang |
| Affiliation: | 1Department of Anesthesiology,Hubei Zhongshan Hospital,Wuhan 430033,China 2Department of Pathology,Wuhan Puai Hospital,Wuhan 430030,China 3Department of Immunology,Medical College of Wuhan University of Science and Technology, Wuhan 430086,China |
| Abstract: | Objective To evaluate the effect of Remifentanil on the expression of bcl-2 and Caspase-3 in rats cerebral cortex following ischemia-reperfusion injury.Methods The cerebral ischemia-reperfusion model was established by thread embolism of the middle cerebral artery.Immunohistochemistry,real-time PCR and neurological evaluation were used to examine the expression of bcl-2 and Caspase-3 in cerebral cortex and neurological function disorders.Results As compared with ischemia-reperfusion group,the relative mRNA level of bcl-2 was increased significantly(P<0.05),that of Caspase-3 was decreased significantly(P<0.05),and neurological function disorders were markedly relieved in Remifentanil group.Conclusion Remifentanil has a neuropretective effect on ischemia-reperfusion injury by increasing the expression of bcl-2,decreasing the expression of Caspase-3 and improving neurological symptoms. |
| Keywords: | Remifentanil bcl-2 Caspase-3 |
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