Antibody and T cell recognition of the light chain of botulinum neurotoxin A in two high-responder mouse strains |
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Authors: | Atassi M Zouhair Oshima Minako Dolimbek Behzod Z Aoki K Roger |
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Affiliation: | a Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA b Department of Immunology, Baylor College of Medicine, Houston, TX, USA c Allergan, Inc., Irvine, CA, USA |
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Abstract: | We investigated in two inbred mouse strains the submolecular recognition of botulinum neurotoxin type A (BoNT/A) by Abs (B cells) and by T lymphocytes. For mapping, we employed a set of overlapping synthetic peptides that encompassed the entire light (L) chain of BoNT/A. After 3 BoNT/A toxoid injections, BALB/c T cells responded in vitro to challenge by peptides L18 (residues 239-257), L23 (309-327), L27 (365-383), L29 (393-411), or L31 (421-439) and more weakly to peptides L3 (29-47), L9/L10 (113-145), L15 (197-215), L17 (225-243), or L26 (351-369). The other peptides stimulated little or no T cell responses. SJL mice mounted, after 3 BoNT/A injections, stronger T cell responses that were medium-to-strong to peptides L2/L3 (15-47), L10/L11 (127-159), L19 (253-271), or L23 (309-327) and low to peptides L17 (225-243), L21 (281-299), L27 (365-383), or L30/L31 (407-439). After 3 BoNT/A injections, BALB/c and SJL antisera protected mice against lethal BoNT/A doses, but displayed restricted epitope profiles compared to outbred (ICR) mice Abs. BALB/c Abs displayed medium-to-high binding to peptides L4/L5 (43-75), L10/L11 (127-159), L18 (239-257) or L27 (365-383). SJL Abs were high to peptides L4/L5 (43-75), L14 (183-201), L16 (211-229), or L18/L19 (239-271), and medium to peptides L10 (127-145), L11 (141-159), L12 (155-173) or L29 (393-411). The other peptides had little or no binding. Responses to each T cell or Ab epitope were under separate genetic control. T and B (antibody) cell recognition regions may coincide, but there were also regions recognized only by Abs or by T cells. |
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Keywords: | Ab, Antibody BoNT, Botulinum neurotoxin BoNT/A, Botulinum neurotoxin serotype A BoNT/B, Botulinum neurotoxin serotype B BSA, Bovine serum albumin L chain, The light chain (residues 1-448) of BoNT/A H chain, The heavy chain (residues 449-1296) of BoNT/A LNC, Lymph node cells PBS, 0.15 M NaCl in 0.01 M sodium phosphate buffer, pH 7.20 SI, Stimulation index = mean cpm incorporated by stimulated cells/mean cpm incorporated by unstimulated cells |
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