Inhibition of 2-acetylaminofluorene oxidations by monoclonal antibodies specific to 3-methylcholanthrene-induced rat liver cytochrome P450 |
| |
| Authors: | S S Thorgeirsson N Sanderson S S Park H V Gelboin |
| |
| Affiliation: | 1Laboratory of Carcinogen Metabolism, National Cancer Institute NIH, Bethesda, MD 20205, USA
2Laboratory of Molecular Carcinogenesis, Division of Cancer Cause and Prevention, National Cancer Institute NIH, Bethesda, MD 20205, USA |
| |
| Abstract: | The inhibitory effects of ten monoclonal antibodies specificfor methylcholanthrene (MC)-induced P450 were determined onboth C- and N-hydroxylations of 2-acetylaminofluorene (AAF)by liver microsomes from rats pretreated with MC, phenobarbitalas well as untreated animals. The inhibitory effects of theseantibodies were also determined on AAF hydroxylations by purifiedrat liver MC-P450. The inhibition patterns of AAF hydroxylationswith both microsomes and the purified P450 indicate that theformation of 7-OH-, 5-OH-, 3-OH-, 1-OH- and N-OH-AAF was catalyzedby the same unique isoenzyme or at least isoenzymes of commonantigenic determinants. |
| |
| Keywords: | |
| 本文献已被 PubMed Oxford 等数据库收录！ |
|
