| 参附对急性肺损伤大鼠热休克蛋白70表达的影响 |
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| 引用本文： | 王曾庚,聂祥碧,郭经华,杨小刚,杨春丽.参附对急性肺损伤大鼠热休克蛋白70表达的影响[J].南昌大学学报(医学版),2011(10). |
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| 作者姓名： | 王曾庚 聂祥碧 郭经华 杨小刚 杨春丽 |
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| 作者单位： | 江西省人民医院ICU; |
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| 摘 要： | 目的观察参附对急性肺损伤(ALI)大鼠热休克蛋白70(HSP70)表达的影响,初步探讨参附对内毒素致急性肺损伤的保护作用机制。方法采用尾静脉注射内毒素建立急性肺损伤模型,将SD大鼠按随机数字表法分为生理盐水对照组(NS组)、内毒素注射组(ET组)、参附治疗组(SF组),每组10只。参附采用腹腔注射给药,分别于尾静脉注射内毒素或生理盐水后2h处死实验动物。观察肺组织病理学检查及评分、肺系数和动脉血氧分压(PaO2),并应用免疫组织化学和蛋白印迹方法检测肺组织中HSP70的表达。结果 ET组较NS组肺组织病理学检查评分、肺系数和HSP70表达显著升高(P<0.05),而PaO2显著下降(P<0.05);SF组和ET组相比,其肺组织病理学检查评分和肺系数显著降低(P<0.05),PaO2和肺组织HSP70表达显著升高(P<0.05)。结论参附可显著减轻内毒素所致急性肺损伤,同时显著增加肺组织HSP70的表达。
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| 关 键 词： | 急性肺损伤 热休克蛋白70 参附注射液 动物 实验 大鼠 |
Effect of Shenfu on Heat Shock Protein 70 Expression in Rats with Acute Lung Injury |
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| WANG Zeng-geng,NIE Xiang-bi,GUO Jing-hua,Yang Xiao-gang,Yang Chun-li.Effect of Shenfu on Heat Shock Protein 70 Expression in Rats with Acute Lung Injury[J].Journal of Nanchang University(Medical Science),2011(10). |
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| Authors: | WANG Zeng-geng NIE Xiang-bi GUO Jing-hua Yang Xiao-gang Yang Chun-li |
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| Institution: | WANG Zeng-geng,NIE Xiang-bi,GUO Jing-hua,Yang Xiao-gang,Yang Chun-li(ICU,Jiangxi Provincial People's Hospital,Nanchang 330006,China) |
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| Abstract: | Objective To observe the effect of shenfu on heat shock protein 70(HSP70) expression in rats with acute lung injury(ALI),and to investigate the mechanism of protective effect of shenfu on endotoxin-induced ALI.Methods The endotoxin was injected into rats via the caudal vein to induce ALI and shenfu was given by intraperitoneal injection.The SD rats were randomly divided into there groups: normal saline group(NS group),endotoxin injection group(ET group) and shenfu treatment group(SF group),with 10 rats in e... |
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| Keywords: | acute lung injury heat shock protein 70 shenfu injection animals laboratory rats |
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