A survey of Neospora caninum-associated bovine abortion in large dairy farms of Mashhad, Iran |
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Authors: | G R Razmi H Zarea Z Naseri |
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Institution: | (1) Department of Pathobiology, School of Veterinary Medicine, Mashhad, Iran;(2) Excellence Research Center for Ruminantal Abortion and Neonatal Mortality, Ferdowsi University of Mashhad, Mashhad, Iran;(3) Present address: Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, P.O. Box: 91775-1793, Iran |
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Abstract: | Neospora caninum is an apicomplexan protozoon causing abortion in cattle worldwide. The present study was designed to assess the importance
of this parasite for causing abortion in dairy farms in the Mashhad area of Iran. Of the aborted bovine fetuses, 151 were
collected from dairy farms between 2006 and 2008. First, brain samples were examined by polymerase chain reaction (PCR) for
the presence of N. caninum DNA, diagnosis was complemented with immunohistochemistry (IHC) and fetal serology (ELISA). Twenty-two (14.5%) of bovine
fetuses were considered to be infected with N. caninum with at least one diagnostic technique being positive. PCR yielded 18 (11.9%) positive out of 151 brain samples. Only 52
brain samples were suitable for IHC examination, and N. caninum organism was detected in six (11.5%) of these 52 brain samples. Fetal fluids (n = 151) were assessed with a N. caninum-ELISA, resulting in 15 (9.9%) seropositive fetal fluids samples. In the present study, a good agreement was observed between
PCR and ELISA, and a fair agreement between PCR and IHC. The results indicated that abortion due to N. caninum infection is prevalent among large-size dairy farms in the Mashhad area of Iran, and that different complementary diagnostic
techniques should be used to increase the chance to detect N. caninum. |
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