Evaluation of twenty rapid antigen tests for the detection of human influenza A H5N1, H3N2, H1N1, and B viruses |
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| Authors: | Janette Taylor Kenneth McPhie Julian Druce Chris Birch Dominic E Dwyer |
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| Institution: | 1. Center for Virus Research, Westmead Millennium Institute, Westmead Hospital, Westmead, New South Wales, Australia;2. Center for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Department of Virology, Westmead Hospital, Westmead, New South Wales, Australia;3. Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia |
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| Abstract: | Twenty rapid antigen assays were compared for their ability to detect influenza using dilutions of virus culture supernatants from human isolates of influenza A H5N1 (clade 1 and 2 strains), H3N2 and H1N1 viruses, and influenza B. There was variation amongst the rapid antigen assays in their ability to detect different influenza viruses. Six of the 12 assays labeled as distinguishing between influenza A and B had comparable analytical sensitivities for detecting both influenza A H5N1 strains, although their ability to detect influenza A H3N2 and H1N1 strains varied. The two assays claiming H5 specificity did not detect either influenza A H5N1 strains, and the two avian influenza‐specific assays detected influenza A H5N1, but missed some influenza A H3N2 virus supernatants. Clinical trials of rapid antigen tests for influenza A H5N1 are limited. For use in a pandemic where novel influenza strains are circulating (such as the current novel influenza A H1N1 09 virus), rapid antigen tests should ideally have comparable sensitivity and specificity for the new strains as for co‐circulating seasonal influenza strains. J. Med. Virol. 81:1918–1922, 2009. © 2009 Wiley‐Liss, Inc. |
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| Keywords: | influenza rapid antigen detection |
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