Co‐production of γ ‐glutamylcysteine and glutathione by mutant strain Saccharomyces cerevisiae FC‐3 and its kinetic analysis |
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| Authors: | When‐Chang Chen Dr. Fu‐Kuo Huang Shu‐Chuan Cheng Feng‐Yuan Tsai Chung‐Liang Lin |
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| Affiliation: | 1. Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan;2. Solvent & Chemical Business Division, Taiwan CPC Corporation, Chiayi, Taiwan |
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| Abstract: | Co‐production of γ ‐glutamylcysteine (γ ‐GC) and glutathione (GSH) by a novel mutant strain Saccharomyces cerevisiae FC‐3 and its kinetic analysis were investigated. The strain could produce γ ‐GC and GSH with high yields (4.22 and 14.3 mg/g‐DCW, respectively) in batch submerged cultures. Effects of medium components and cultivation conditions on cell growth and the contents of intracellular γ ‐GC and GSH were examined. Results show that 2% (w/v) sucrose and 2.5% (w/v) yeast extract are the best carbon and nitrogen sources, respectively, and supplement of three amino acids (glycine, cysteine and glutamate), each at 0.08% (w/v), in the medium could enhance γ ‐GC and GSH production. In addition, optimal operating conditions are at the initial pH value of 7.0, 30 °C and 200 rev/min. Moreover, results obtained from kinetic analyses reveal that γ ‐GC production is mixed‐type growth associated, but GSH production is growth‐associated. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
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| Keywords: | Co‐production γ ‐Glutamylcysteine Glutathione Saccharomyces cerevisiae Maximization Kinetic analysis |
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