| 针对livin的siRNA表达载体的构建 |
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| 引用本文： | 刘敏丽,魏晓丽,张生军,崔轶霞,杜雨柔,韩振奎.针对livin的siRNA表达载体的构建[J].延安大学学报(医学科学版),2012,10(1):4-6,19. |
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| 作者姓名： | 刘敏丽 魏晓丽 张生军 崔轶霞 杜雨柔 韩振奎 |
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| 作者单位： | 1. 延安大学医学院病理学教研室
2. 延安大学附属医院,陕西延安71600 |
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| 摘 要： | 目的设计能够有效抑制livin基因表达的小干扰RNA，构建针对livin基因的siRNA重组表达载体。方法通过分子克隆技术，设计并合成具有基因特异性的一组寡核苷酸片段，克隆到pSilencerTM3．1-H1hygro载体，并经BamHI／EeoRI双酶切及测序鉴定证实，livin的siRNA序列成功地克隆到表达载体中。结果经BarnHI／EcoRI双酶切及测序鉴定证实，人livin的siRNA序列成功地克隆到表达载体pSilencerTM3．1．H1hygro中，构建了表达载体，测序分析证实插入序列正确。结论成功构建了livin表达载体，为后续研究奠定基础。
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| 关 键 词： | livin 表达载体 小干扰RNA |
Construction livin-specific siRNA expression vector |
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| LIU Min-li
,
WEI Xiao-li
,
ZHANG Sheng-jun
,
CUI Yi-xia
,
DU Yu-rou
,
HAN Zhen-kui.Construction livin-specific siRNA expression vector[J].Journal of Yanan University:Medical Science Edition,2012,10(1):4-6,19. |
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| Authors: | LIU Min-li WEI Xiao-li ZHANG Sheng-jun CUI Yi-xia DU Yu-rou HAN Zhen-kui |
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| Institution: | 1(1.Department of Pathology,Medical College of Yan’an University,2.Yan’an University Affiliated Hospital,Shaan xi Yan’an,716000,China.) |
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| Abstract: | Objective The study aims to design a siRNA which could inhibit livin gene and construct eukaryotic expression vector.Methods A group of double strand oligonucleotide fragments were synthesized and cloned into pSilencerTM 3.1-H1 hygro vector with molecular cloning technique.It was confirmed by restrictive enzymes(BamHⅠ/EcoRⅠ)digestion and analysis of DNA sequencing.Results Restrictive enzymes digestion analysis and DNA sequencing encoding livin was exactly the same as the reported sequence of encoding human livin in GenBank.The recombinant plasmid was constructed.Conclusion The expression vector of livin were constructed successfully which lay the foundation for next study. |
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| Keywords: | livin expression vectors Small interfering RNA |
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