Isolation of polymorphic microsatellite loci from an endangered freshwater species Chinese sucker, Myxocyprinus asiaticus |
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| Authors: | Nan Chen Huan Ling Wang Wei Min Wang Vibeke Simonsen |
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| Affiliation: | 1.Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery,Huazhong Agricultural University,Wuhan,People’s Republic of China;2.National Environment Research Institute,Silkeborg,Denmark |
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| Abstract: | Chinese sucker (Myxocyprinus asiaticus) is an endangered freshwater fish species endemic to the Yangtze River in China. In this work, we described the isolation and characterization of 14 new polymorphic microsatellite loci from (CA) n and (GA) n microsatellite enriched libraries of the M. asiaticus. The number of alleles per locus ranged from 6 to 14 with the observed (H O) and expected (H E) heterozygosities ranging from 0.120 to 1.000 and from 0.767 to 0.952, respectively. Five loci displayed significant departures from Hardy–Weinberg expectations. These polymorphic microsatellite loci will be useful molecular tools for understanding population genetic structure and conservation of M. asiaticus. |
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