| 正常人肾上腺组织基因表达谱的研究 |
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| 引用本文： | 胡仁明,彭永德,任双喜,顾彦杰,宋怀东,黄长晖,傅肃能,李月彬,黄秋花,张成康,顾健,俞亚萍,徐淑华,傅刚,陈名道,韩泽广,陈家伦. 正常人肾上腺组织基因表达谱的研究[J]. 中华内分泌代谢杂志, 2000, 16(1): 10-14 |
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| 作者姓名： | 胡仁明 彭永德 任双喜 顾彦杰 宋怀东 黄长晖 傅肃能 李月彬 黄秋花 张成康 顾健 俞亚萍 徐淑华 傅刚 陈名道 韩泽广 陈家伦 |
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| 作者单位： | 1. 200025,上海第二医科大学附属瑞金医院内分泌科,上海市内分泌研究所
2. 国家人类基因组南方研究中心 |
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| 基金项目： | 国家人类基因组南方中心资助项目(CNCS-98m-11);国家自然科学基金资助项目(39870311);卫生部科研基金资助项目(98-2-285) |
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| 摘 要： | 建立正常人肾上腺组织基因表达目录。方法肾上腺文库构建、大规模表达序列标签（ＥＳＴｓ）测序及生物信息学分析。结果基因／蛋白表达类的基因在肾上腺组织表达最高,代谢类基因次之。前３位高表达基因均为类固醇激素合成酶类及相关蛋白。肾上腺表达ＣＲＨ、尿皮素（ＣＲＨ相关蛋白）黑色素浓集素（ＭＣＨ）、可卡因和安菲他明调节转录物（ＣＡＲＴ）及垂体腺苷酸环化酶活化多态（ＰＡＣＡＰ）等激素及各类受体,包括细胞因子,神经
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| 关 键 词： | 肾上腺 基因表达 表达序列标记 计算生物学 |
| 修稿时间： | 1999-07-14 |
Investigation of the profilings of the gene expression in normal human adrenal tissue |
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| HU Renming ,PENG Yongde,REN Shuangxi,et al.. Investigation of the profilings of the gene expression in normal human adrenal tissue[J]. Chinese Journal of Endocrinology and Metabolism, 2000, 16(1): 10-14 |
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| Authors: | HU Renming PENG Yongde REN Shuangxi et al. |
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| Affiliation: | HU Renming *,PENG Yongde,REN Shuangxi,et al. *Shanghai Institute of Endocrinology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025 |
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| Abstract: | Objective To establish the gene expression profiles in human normal adrenal glands (AD). Methods cDNA libraries of human normal adrenal gonads were constructed. Large scale sequencing and bioinformatics were used. Results The category of genes highly expressed in AD was gene/protein expression, followed by metabolism. The first three genes highly expressed in AD were the enzymes and protein involved in the biosynthesis of steroids. Hormones or secretory proteins, such as CRH, MCH, urocortin, CART (cocaine and amphetamine regulated transcript) and PACAP (pituitary adenylate cyclase activating polypeptide) were expressed in AD. Receptors of cytokines, neuropeptides and neurotransmitters were also expressed in AD. The synthases of steroids, low density lipoprotein receptor, high density lipoprotein binding protein and cholesterol were expressed in AD. Conclusions The genes highly expressed in AD are closely related to the specific functions of AD. Besides steroids, many peptide hormones and cytokines are also expressed in AD. The other systems in human body are extensively associated with AD by the receptors. It is suggested that there may be a local CRH ACTH cortisol regulatory network in AD. |
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| Keywords: | Adrenal glands Gene expression Expressed sequence tags Computational biology |
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