杆菌肽脂质体的制备及其药物含量测定

目的：制备杆菌肽脂质体,并采用HPLC法测定杆菌肽脂质体中杆菌肽含量和包封率。方法用薄膜分散法将杆菌肽制备成杆菌肽脂质体；色谱条件：色谱柱为Diamonsil-C18色谱柱（150mm×4.6mm，5μm）；流动相为pH 6.0的磷酸盐缓冲液-甲醇（60∶40）；柱温为25℃；检测波长为254nm；流速为1mL/min。采用低温离心法分离杆菌肽脂质体中的游离药物。结果所制备的杆菌肽脂质体的平均包封率为87.94%（RSD 1.12%, n=5）；在本色谱条件下，杆菌肽在0.061~1.83μg范围内与峰面积积分值的线性关系良好（R2=0.9997），平均回收率为99.68%~101.38%。结论本HPLC法简单，准确可靠，可用于杆菌肽脂质体中杆菌肽含量及其包封率的测定。

Preparation and the drug content determination of bacitracin Liposom

Objective To prepare bacitracin liposom,and to determine bacitracin content and entrapment efficiency of the liposome using HPLC method. Methods The bacitracin liposome was prepared by film dispersion method; The chromatographic conditions were Column： Diamonsil-C18 column（150mm×4.6mm,5μm）; mobile phase： pH 6.0 phosphate buffer： methanol （60∶40）; column temperature： 25℃; detection wavelength： 254nm; flow rate： 1mL/min.Refrigerated centrifugation using bacitracin liposomes free drug. Results The mean encapsulation efficiency of the bacitraci liposome was 87.94%（RSD 1.12%,n=5）;In the chromatographic conditions bacitracin and accessories and a good separation of the solvent peak,bacitracin at 0.061-1.83μg range had a good linear relationship （R2=0.9997）,recovery was 99.68%-101.38%. Conclusion The HPLC method is simple, accurate and reliable,can be used to determine bacitracin content and entrapment efficiency of the bacitracin liposom.