| 密度梯度离心法提纯Coxsackie B_3病毒的实验研究 |
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| 引用本文： | 张福明,张淑琴,张鸿源. 密度梯度离心法提纯Coxsackie B_3病毒的实验研究[J]. 吉林大学学报(医学版), 1992, 0(4) |
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| 作者姓名： | 张福明 张淑琴 张鸿源 |
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| 作者单位： | 白求恩医科大学应用基础医学研究所(张福明，张淑琴)，白求恩医科大学应用基础医学研究所(张鸿源) |
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| 摘 要： | 本文用庶糖密度梯度离心法提纯Coxsackie B_3病毒。用不同转子、不同转速和不同时间离心提取Coxsackie B_3病毒,病毒感染性试验检测结果表明,用S-27-2转子(约87400g)离心3h结果最佳,所得列的A、B、C3条区带中,C带病毒毒力较A、B两带可提高10~(8-10)倍。本实验方法在小RNA病毒提纯方面有参考价值,为Coxsackie B组病毒的分子病毒学研究提供了一种新的手段。
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| 关 键 词： | 柯赛奇病毒 密度梯度离心 |
STUDY ON PURIFICATLCN OF COXSACKIE B_3 VIRUS BY DENSITY GRADIENT CENTRIFUGATION |
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| Zhang Fuming,Zhang Shuqin,Zhang Hongyuan. STUDY ON PURIFICATLCN OF COXSACKIE B_3 VIRUS BY DENSITY GRADIENT CENTRIFUGATION[J]. Journal of Jilin University: Med Ed, 1992, 0(4) |
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| Authors: | Zhang Fuming Zhang Shuqin Zhang Hongyuan |
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| Affiliation: | Institute of Applied Preclinical Medical Sciences |
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| Abstract: | We purified Coxsackie B3 virus by using sucrose-density-gradient-centrifugation and adoptation of various rotors , speeds and different duration of time. The viral infection test showed that the best result was observed when S-27-2 rotor (about 87400g) was used and centrifugation was continued for 3 hours. Three bands (A, B and C) were obtained and the viulence of band C was 108-10 times stronger than that of band A and B. This experimental method is of referential value in purification of picornavirus. It is also a new method for molecular virological research on Coxsackie B virus. |
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| Keywords: | Coxsackie virus Density gradient centrifugation |
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