| β-防御素3在人精子上的表达及功能研究 |
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| 引用本文： | 刁瑞英,牟丽莎,陈浩,蔡学泳. β-防御素3在人精子上的表达及功能研究[J]. 深圳中西医结合杂志, 2014, 0(5): 1-4 |
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| 作者姓名： | 刁瑞英 牟丽莎 陈浩 蔡学泳 |
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| 作者单位： | 深圳I市第二人民医院,深圳市肿瘤基因组临床应用工程实验室,广东深圳518035 |
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| 基金项目： | 国家自然科学基金青年科学基金项目（81200465）; 深圳市科技计划项目（201203027） |
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| 摘 要： | 目的:探讨附睾上皮分泌的β-防御素3(HBD-3)在人精子上的表达和功能,以及是否参与弱精子症的发生。方法:采用间接免疫荧光染色和Western blot检测HBD-3在人精子的定位和表达;采用计算机辅助精子分析(CASA)检测HBD-3是否参与精子活力和前向运动能力。结果:HBD-3在弱精子症精子的表达量较正常生育组下调。精子上HBD-3表达水平与精子活力呈正相关性。抗HBD-3抗体将干扰正常精子活力,而rHBD-3可改善弱精子症精子的活力。结论:在男性生殖系统中,精子表面HBD-3参与精子活力和前向运动能力发生。本研究为男性不育症提供新型诊断靶点和治疗策略。
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| 关 键 词： | 精子 β-防御素3 前向活力 弱精子症 男性不育症 |
Characteristics and Function of HBD-3 in Human Sperm |
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| DIAO Rui-ying,MOU Li-sha,CHEN Hao,CAI Xue-yong. Characteristics and Function of HBD-3 in Human Sperm[J]. Shenzhen Journal of Integrated Traditional Chinese and Western Medicine, 2014, 0(5): 1-4 |
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| Authors: | DIAO Rui-ying MOU Li-sha CHEN Hao CAI Xue-yong |
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| Affiliation: | (Shenzhen Second People＇s Hospital, Shenzhen Tumor Genomie Clinical Engineering Laboratory, Guangdong Shenzhen 518035) |
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| Abstract: | Objective To investigate the expression and function of HBD-3 secreted by epididymis in human sperm and the association with asthenospermia. Methods The localization and expression of HBD-3 were detected using indirect immunostaining and Western blot. CASA was adopted to determine the function of HBD-3 in human sperm. Results HBD-3 is significantly down-regulated in asthenospermia with both reduced sperm motility （P 〈 0.05）. Interference with HBD-3 function decreases sperm motility in normal sperm, while treatment with recombinant HBD-3 significantly restores HBD-3 functions in sperm from asthenospermia patients. Conclusion The present finding reveals the function of HBD-3 on sperm motlity and forward motility. It also provides novel diagnostic targets and a feasible treatment method for related male infertility. |
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| Keywords: | Sperm HBD-3 Forward motility Asthenospermia Infertiltiy |
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