| 地塞米松对小梁细胞生长周期的影响 |
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| 引用本文： | 卓业鸿,葛坚,林明楷,蓝育青,郑健.地塞米松对小梁细胞生长周期的影响[J].眼科学报,2001,17(2):103-105. |
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| 作者姓名： | 卓业鸿 葛坚 林明楷 蓝育青 郑健 |
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| 作者单位： | 中山医科大学中山眼科中心,广州,510060 |
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| 基金项目： | 国家自然科学基金(39800163),教委博士点基金(9856),省科委自然科学基金(980112) |
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| 摘 要： | 目的：探讨地塞米松对小梁细胞生长周期的影响。方法：按常规方法培养人眼小梁细胞，然后分为二组：常规DMEM培养液及含10^-7M地塞米松的DMEM培养液组，培养3d、5d、7d后，应用流式细胞仪检测细胞周期各阶段G1、S、G2期情况。结果：在正常未用地塞米松处理组，G2期细胞为2．0％；地塞米松处理3、5、7d后，G2期细胞比例各为8．0％、16．3％、19．87％，其差异有显著性意义。结论：地塞米松可使小梁细胞生长周期中G2期的比例增加，提示这种作用可能与激素性青光眼的发病 有关。
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| 关 键 词： | 地塞米松 小梁细胞 细胞周期 激素性青光眼 |
Influence of Cell Cycle in Human Trabecular CellsInduced by Dexamethesone |
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| Y Zhou,J Ge,M Lin,Y Lan,J Zheng.Influence of Cell Cycle in Human Trabecular CellsInduced by Dexamethesone[J].Eye Science,2001,17(2):103-105. |
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| Authors: | Y Zhou J Ge M Lin Y Lan J Zheng |
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| Institution: | Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. |
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| Abstract: | PURPOSE: To investigate the influence of dexamethesone (Dex) on cell cycle in human trabecular cells(HTC). METHODS: The trabecular specimens from human eyes were cultured in vitro. Then the cultured cells were immersed in DMEM solution containing 10(-7) M dexamethesone for three, five and seven days. The cell cycle was detected by Flow Cycometry. RESULTS: The G2 phase of cell cycle in HTC increased when HTC were treated by Dex. The G2 phase was 2% in control group. For three, five and seven days treated by Dex, the G2 phase were 8%, 16.3 and 19.4%. CONCLUSION: The influence of cell cycle might be induced by Dex, it may be related with the pathogenesis of glucocorticoid induced glaucoma. |
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| Keywords: | dexamethesone trabecular cells cell cycle |
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