临产前后胎盘组织bcl-2、Fasl的表达与母血、羊水EGF变化的关系

目的探讨临产前后bc l-2、Fasl在胎盘滋养层细胞的蛋白表达变化及其与母血、羊水中表皮生长因子(EGF)的浓度之间的关系。方法53例足月妊娠妇女分成两组,其中临产组28例,未临产组25例。用免疫组化法测定胎盘滋养层细胞bc l-2、Fasl的蛋白表达,用放射免疫法测定孕妇母血、羊水中EGF的浓度。结果(1)临产组bc l-2、Fasl的表达明显低于未临产组(P<0.05)。(2)临产组母血、羊水EGF水平高于未临产组(P<0.05)。(3)母血、羊水中EGF的变化与bc l-2的表达呈负相关,而与Fasl的表达不相关。结论bc l-2、Fasl介导的胎盘滋养层细胞凋亡在启动分娩发动过程中具有重要作用;EGF可能对bc l-2的表达存在下调作用。

A relationship between the expressions of bcl-2 and Fasl in placental and the concentrations of EGF in maternal blood and amniotic fluid on labor onset

Objective: To investigate the expressions of bcl2 and Fasl proteins in placental trophoblastic cells on labor onset and their relationships with the concentrations of Epidermol Growth Factors(EGF) in maternal blood and amniotic fluid.Methods: Fifty-three women on term pregnancy were divided into two groups.One group on the time of labor onset were twenty-eight women.Another group not on the time of labor onset were twenty-five women.Expressions of bcl-2 and Fasl proteins in placental trophoblastic cells were detected by immunohistochemical assay.The concentrations of EGF in maternal blood and amniotic fluid were detected by radioimmune assay.Results:(1) Expressions of bcl-2 and Fasl in labor onset group were significantly lower than those of the group not labor onset(P<0.05).(2) The concentrations of EGF in maternal blood and amniotic fluid in labor onset group were higher than those of not labor onset(P<0.05).(3) The concentrations of EGF in maternal blood and amnionotic fluid had a negative correlation with the expression of bcl-2,but had not correlation with Fasl.Conclusions: It was considered that the apoptosis of placental trophoblastic cells induced by bcl-2 and Fasl may play an important role in the initiation of labor onset.And EGF may had a down-regulation to the expression of bcl-2.