转基因树突状细胞瘤苗诱导的CTL对肿瘤细胞的靶向性杀伤作用

目的：观察mAFP基因转染的树突状细胞（DC）瘤苗诱导的靶向性细胞毒性T细胞（以下简称靶向性CTL）对肿瘤细胞的杀伤作用。方法：用构建的mAFP基因转染的DC瘤苗免疫C57BL／6小鼠，取免疫小鼠脾细胞诱导CTL，LDH非放射性细胞毒性试验检测其对不同肿瘤细胞的杀伤作用。用ELISA法检测CTL诱导过程中TNF—α和IFN-γ分泌变化规律。结果：AFP基因转染的DC瘤苗免疫小鼠后能诱导具有较高抗肿瘤活性的靶向性CTL，这种靶向性CTL对AFP分泌性肿瘤细胞具有更强的杀瘤活性，且在CTL诱导过程中TNF-α和IFN-γ分泌水平明显增高，与对照组比较差异有统计学意义（P〈O．05）。结论：AFP基因重组腺病毒修饰的DC瘤苗免疫C57BL／6J小鼠，能诱导出针对AFP抗原的靶向性CTL，并也能通过分泌TNF-α和IFN-γ实现对AFP分泌性肿瘤细胞的特异性杀伤。

THE CYTOLYTIC EFFECT OF TARGETING CYTOTOXIC TLYMPHOCYTE INDUCED BY TRRANSGENIC DENDRITIC CELLS TUMOR VACCINE

Objective： To investigate the cytolytic effect of targeting cytotoxic T lymphocyte induced by transgenie dendritic cells （DC） tumor vaccine. Methods：C57BL/6 mice were inoculated with DC tumor vaccine which was engineered with murine alpha fetoprotein （mAFP） gene. Targeting cytotoxic T lymphoeytes （CTL） was induced from spleen cells of inoculated mice and cytolytic effect was tested by LDH nonirradiated eytotoxic test. TNF-α and IFN-γ secretion level were detected with ELISA method. Results：The targeting CTL which was induced by AFP antigen has more effective eytolytic effect against AFP-secreting tumor cells than the control group, and there was remarkably significance for TNF-α and IFN-γ secretion level of the experimental group than the control group. Conclusion： Targeting CTL could be induced by transgenic DC tumor vaccine which was engineered with mAFP gene and specifically kill the tumor cells with AFP-secreting, its cytolitic effect is also related to higher level of TNF-α and IFN-γ secretion.