| Affiliation: | 1. Department of Pathology, Addenbrooke's Hospital, Cambridge University NHS Foundation Trust, Cambridge, UK;2. Haematopathology and Oncology Diagnostics Service, Addenbrooke's Hospital, Cambridge University NHS Foundation Trust, Cambridge, UK Clinical Pathology Department, University of Cairo, Cairo, Egypt;3. Haematopathology and Oncology Diagnostics Service, Addenbrooke's Hospital, Cambridge University NHS Foundation Trust, Cambridge, UK;4. Department of Pathology, University of Cambridge, Cambridge, UK |
| Abstract: | Identification of clonal IGH, IGK and IGL gene rearrangements offers diagnostic adjunct in suspected B-cell neoplasms. However, many centres omit IGL analysis as its value is uncertain. A review of 567 cases with IGH, IGK and IGL rearrangement assessed using BIOMED-2 assays showed clonal immunoglobulin gene rearrangement in 54% of cases, of which 24% had a clonal IGL rearrangement. In two cases, the clonal rearrangement was detected exclusively by IGL analysis. This finding demonstrates the added value of IGL analysis for clonality assessment, especially in suspected B-cell neoplasms in which a clonal IGH and/or IGK rearrangement is not detected or is equivocal. |
