胆总管内注射无水乙醇致胆道闭锁动物模型的建立

目的 应用胆总管内注射无水乙醇建立SD大鼠胆道闭锁模型. 方法 将SD雄性大鼠随机分为实验组和对照组，在实验组中经静脉留置针插入胆总管注入无水乙醇，对照组注入生理盐水.观察SD大鼠的生化及病理结果. 结果 在实验组中SD大鼠根据病理及生化检测分为肝功能持续恶化组和肝功能修复组，肝功能持续恶化组在8周以后生化明显高于对照组及肝功能修复组.常规HE染色及SMA、Masson染色也出现明显变化.结论 胆总管无水乙醇注射诱导胆道闭锁模型是一种可靠的动物模型，此动物模型会帮助人们进一步研究胆道闭锁提供更多的研究手段.

Injection of ethanol into the common bile duct to establish a rat model of biliary atresia

Objective To establish a new rat model of biliary atresia by pure ethanol injection into the common bile duct. Methods A catheter was inserted and fixed in the common bile duct in male SD rats. Saline (8 rats) or pure ethanol (16 rats) was injected through the catheter,respectively, and the biochemical and pathological changes in the rats were examined. Results SD rats in the experimental group were divided into a persistent injury and a restoration of liver dysfunction groups according to pathological and biochemical detection. In the persistent injury group, biochemical impairments were significantly higher at 8 weeks after ethanol injection than those in the control group and restoration group. Distinct pathological changes in the liver were observed using HE, SMA, and Masson staining. Conclusions It is a reliable animal model of biliary atresia induced by injection of pure ethanol into the common bile duct in the rat. It will provide a useful tool in future studies of biliary atresia.