小鼠Lewis肺癌原位模型的建立

目的 利用Matrigel与Lewis制备细胞混悬液注射于小鼠左肺内，建立小鼠Lewis肺癌原位模型，评价其肿瘤生长情况、转移情况，以期建立更稳定、更接近于人肺癌生长情况的小鼠肺癌原位模型。方法 将处于对数生长期的Lewis肺癌细胞混悬于Matrigel中，接种于C57BL/6近交系小鼠左肺内。分别于第4,7,10,13,16天各处死5只小鼠，观察其局部成瘤率、肿瘤生长情况、中位生存期及肿瘤转移情况，并对各阶段小鼠行肺部，肝脏，肾脏，脾脏病理切片检查。结果 术后第7天解剖的5只小鼠中，3只小鼠肺上可见小的瘤结节形成，其余2只肺上未见肉眼成瘤，行病理HE染色检查在显微镜下可见2只小鼠肺脏有小的瘤结节形成。术后第10天以后处死的所有小鼠肺上均有肉眼成瘤，术后第13天，所有小鼠肺原位成瘤并伴有血性胸腔积液、胸腔内转移。术后第25天，有1只小鼠出现上述转移的同时还出现了心包膜转移及肾脏远处转移。5只小鼠生存期分别为17d、20d、22d、22d、25d，小鼠中位生存期为21.2d（17-25d）。成瘤率100%。结论 利用Matrigel法成功建立小鼠Lewis肺癌原位模型，稳定性好，成瘤率高，并具有远处转移的特性，更接近于人肺癌的发生、发展过程，故此方法有进一步推广的价值。

Establishment of a mouse model of orthotopic Lewis lung cancer

Objective To establish a mouse model of orthotopic Lewis lung carcinoma using Matrigel, to evaluate the tumor growth and metastasis, and to provide a more stable mouse model of orthotopic lung cancer, which is more similar to human lung cancer. Methods Logarithmic phase of cultured Lewis lung cancer cells were suspended in Matrigel, vaccinated into the left lung of inbred C57BL/6 mice. Five mice were killed on the 4th, 7th, 10th, 13th, and 16th days, respectively, and to observe the median survival, tumor formation rate, tumor growth, and metastasis. Pathological changes of the mouse lung, liver, kidney and spleen were examined. Results In 5 mice killed on the 7th postoperative day, small tumor nodules were observed on the lung in three mice and no tumor was visible by gross inspection in the other two mice, but small tumor nodules were observed under the microscope. For all the mice killed on the 10th postoperative day, tumors were visible to the naked eye on the lung of all the five mice. On the 13th day, orthotopic tumor was observed on the lung with bloody pleural effusion and pleural metastasis in all the five mice. On the 25th day, in addition to the pleural metastasis, one mouse had pericardial metastasis and renal metastasis. The survival periods of the 5 mice were 17 d, 20 d, 22 d, 22 d, and 25 d, respectively, with a median survival period of 21.2 d (17-25 d), and the tumor formation rate was 100%. Conclusions Mouse models of orthotopic Lewis lung carcinoma is successfully established using injection of tumor cells suspended in Matrigel. This model is more similar to the growth of human lung cancer, with good stability, high tumor formation rate and characteristics of distant metastasis, therefore, is worthy of further application.