特异区段替换小鼠性发育表型的研究

【摘要】 目的 了解性发育相关数量性状基因座（ Quantitative Trait Locus，QTL）（DXMit68-rs29053133）在近交系小鼠A/J 和C3H/HeJ (C3H)中是否存在影响表型的序列差异，以帮助对候选基因进行筛选。方法 利用A/J 和C3H/HeJ (C3H)构建了针对该区段的特异区段替换系小鼠，并对其雌鼠的性发育相关性状进行研究。结果 A/J 和C3H/HeJ在这一QTL中染色体的序列差异，没有引起相关性状的明显差异。结论 研究结果显示，A/J 和C3H/HeJ小鼠中该QTL区段中存在序列差异的基因并不是引起性发育表型产生差异的候选基因。

Study on the pubertal phenotype in Specific Sections Replacement Mouse

Abstract: Objective: This thesis intends to investigate whether there are sequence differences between the inbred strains of mice A / J and C3H/HeJ (C3H) onanalyze a puberty relevant quantitative trait loci locus (Quantitative Trait Locus, QTL) (DXMit68-rs29053133) on whether there are sequence differences affecting the phenotype in order inbred strains of mice A / J and C3H/HeJ (C3H) to help on screening of the candidate genes. Methods: To build a Specific Sections Replacement strain replacement for the region specific section based on the between A / J and C3H/HeJ (C3H) strain mices , and study the puberty traits of their females puberty traits. This QTL chromosome sequence differences in between A / J and C3H/HeJ in this QTL did not cause significant difference in the related traits phenotype. Conclusion: The results of this study showed that the genes with sequence differences in this QTL section of the mice region between A/J and C3H/HeJ are not among the candidate genes that cause the puberty phenotypic differences.