无线遥测和刺激技术建立兔房颤模型的探索

目的 探索一种在无线遥测和刺激技术基础上的兔房颤模型的制作.方法 新西兰兔皮下植入自主研发的植入式遥测刺激器,植入式遥测刺激器的制作是以TI公司(德州仪器)的MSP单片机和TI公司的RF无线收发芯片CC2250为核心开发设计.优化植入系统设计以满足新西兰兔房颤模型建立的探索实验;植入子植入新西兰兔腹部皮下,采集电极留置于左上肢和右上肢腋下皮下,两个刺激电极分别缝合于左心耳和左心房上,通过无线收发采集和刺激信号;实现利用Powerlab生理记录仪连续监测体表I导联心电信号,并通过专用计算机程序刺激软件,发放间歇(刺激2 s,暂停2 s)高频(频率20 Hz)阈上(强度2 mA,脉宽1 ms)刺激,若间歇期内出现房颤,则人为干预中止刺激,若转为窦性心律,则继续刺激.结果 植入式遥测刺激器在体内可稳定工作(包括采集模拟心电信号和发放刺激)30 d,植入新西兰兔体内刺激3周后可诱导出房颤,持续时间>48 h.结论 用新西兰兔代替比格犬建立基于无线遥测和刺激基础上的房颤模型是完全可行的,同时也体现了动物福利优化和替代原则.

Exploratory experimental study on rabbit model of atrial fibrillation by wireless telemetering and stimulation technology

Objective To explore the establishment of a rabbit model of atrial fibrillation by wireless telemetering and stimulation technology. Method An implantable telemetering stimulator which was independently designed and developed was hypodermically implanted in New Zealand rabbits. The implantable telemetering stimulator was made with the core development and design of MSP single-chip microcomputer of TI Corporation (Texas Instruments) and RF wireless transceiver chip CC2250 of TI Corporation. The design of the implantation system was optimized to cater to the exploratory experiment to establish atrial fibrillation model of New Zealand rabbits. The implanter was implanted into the abdominal subcutaneous tissue of the New Zealand rabbits, the collecting electrodes were placed in the oxter subcutaneous tissues of the left and the right upper extremities, and the two stimulating electrodes were sutured at the left auricle and the left atrium. The signals were collected and stimulated by the wireless transceiver. The I-lead ECG electrical signals were continuously monitored on the body surface by a Powerlab physiological recorder. High frequency (>20 Hz) suprathreshold stimulus (intensity 2 mA, pulse width 1 ms) was emitted by specialized stimulation software of a computer program by the interval (stimulating for 2 s and pausing for 2 s). In case of atrial fibrillation during intervals, the stimulation could be stopped by hand. In case of sinus rhythm, the stimulation could be continued. Results The implantable telemetering stimulator can work stably in vivo (including collecting stimulated electrocardio signal and emitting stimulations) for 30 days. Atrial fibrillation can be induced after stimulating in vivo of the New Zealand rabbits for 3 weeks, with a duration of >48 h. Conclusions Applying implantable telemetering stimulator can build a New Zealand istead of beagles model of atrial fibrillation which is more consistent with welfare optimization and substitution principle for laboratory animals.