肠易激综合征大鼠模型的复制与评价

目的 建立肠易激综合征(IBS)大鼠模型,为实验药理学提供方法.方法 采用复合因素诱导大鼠建立IBS模型,测定模型大鼠体重、摄食量、排便情况、自主运动量、胃排空率和肠推进率,分析血清5-HT、血浆SP、VIP含量以及结肠匀浆5-HT、SP、VIP含量变化,测定血液生化指标,显微观察胃窦黏膜和横结肠黏膜组织形态改变.结果 造模后各大鼠体重减轻、摄食量减少,排便量增多、出现稀便和无定形软便,自主运动量减少,胃排空率减小、肠推进率加快,血清5-HT含量升高、血浆SP和VIP含量均降低,结肠匀浆5-HT和SP、VIP含量升高(P<0.05,P<0.01),血液生化指标未见异常,胃窦黏膜和横结肠黏膜无明显形态改变;匹维溴铵15.0 mg/kg治疗30 d后,大鼠体重、摄食量增加,排便量减少、稀便减少,自主运动量接近正常,血清5-HT含量下降、血浆SP和VIP含量升高,结肠匀浆5-HT和SP、VIP含量下降.结论 复合因素随机刺激能成功复制IBS大鼠模型,其病理生理特征与临床研究结果基本吻合.

Replication and evaluation of a rat model of irritable bowel syndrome

Objective The aim of this study was to establish a rat model of irritable bowel syndrome. Methods Thirty healthy adult SD rats (mele:female=1:1) were divided into normal control group, model group, and positive control group (pinaverium bromide tablets 15.0 mg/ kg) for 31 days. Body weight, appetite, defecation, voluntary movement of all the rats were determined. The rates of gastric emptying and small intestinal propulsion rate were measured. The serum 5-HT and plasma SP and VIP or 5-HT, SP, VIP in colon homogenates were assessed by radioimmunoassay. Blood biochemical parameters were measured with an automatic biochemical analyzer. The gastric and intestinal morphology was evaluated by histological examination. Results After modeling, the rat weight and food intake were decreased, ad stool quantity was increased. The voluntary movement and gastric emptying rates were decreased, intestinal propulsion rates were increased, and the contents of SP and VIP in blood were decreased, but increased in the colonic homogenate (P<0.05, P<0.01). After treatment, the food intake was increased and stool quantity was decreased, the rat body weight was significantly increased, the amount of voluntary movement and stool returned near to normal, the 5-HT levels in serum or in colonic homogenate were significantly decreased, but plasma VIP levels were markedly increased, and the SP and VIP contents were significantly decreased in colonic homogenate in the positive control group (P<0.05, P<0.01). Hematology indexes had no obvious changes. The gastric and colonic tissue morphology showed no distinct damages caused by the diverse stimulating factors. Conclusions The stimulation of composite factors can be used to successfully generate the rat model of irritable bowel syndrome, showing similar clinical manifestation of this disease in humans.