| 抑制T细胞增殖的抗CD45单克隆抗体的建立 |
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| 引用本文： | 苏泽红,李亚林,谭翔文,余坚,刘鑫,袁月亲,练高建.抑制T细胞增殖的抗CD45单克隆抗体的建立[J].实验动物与比较医学,2014,22(5):35-38. |
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| 作者姓名： | 苏泽红 李亚林 谭翔文 余坚 刘鑫 袁月亲 练高建 |
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| 作者单位： | 南华大学,南华大学药学与生物科学学院 |
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| 基金项目： | 湖南省科技厅项目（2013TT2007），湖南省教育厅优秀青年项目（13B102），湖南省自然基金（13JJ3081） |
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| 摘 要： | 目的 寻找能调节T细胞功能的相关分子，进行与T细胞介导的自身免疫性疾病相关的研究。方法 从BALB/c小鼠骨髓中收集树突状细胞，免疫Wistar大鼠，进行细胞融合，建立杂交瘤细胞系。筛选得到很多株能调节T细胞功能的杂交瘤细胞系，对其中一株最能抑制T细胞增殖的杂交瘤细胞系进行了进一步的深入研究。结果 显示其目标分子是CD45，同时增殖实验结果显示该抗体能显著抑制T细胞增殖反应。结论 抗CD45单克隆抗体能有效抑制T细胞增殖，有望将本抗体用于T细胞介导的自身免疫性疾病的相关预防及治疗中。
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| 关 键 词： | CD45 单克隆抗体 抑制 T细胞增殖 |
| 收稿时间： | 2014/1/10 0:00:00 |
| 修稿时间： | 2014/4/24 0:00:00 |
Establishment of an anti-CD45 monoclonal antibody that inhibits T-cell proliferation |
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| SU Ze-hong,LI Ya-lin,TAN Xiang -wen,YU-Jian,LIU Xin,YUAN Yue-qin and LIAN Gao-jian.Establishment of an anti-CD45 monoclonal antibody that inhibits T-cell proliferation[J].Laboratory Animal and Comparative Medicine,2014,22(5):35-38. |
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| Authors: | SU Ze-hong LI Ya-lin TAN Xiang -wen YU-Jian LIU Xin YUAN Yue-qin and LIAN Gao-jian |
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| Institution: | Department of Biotechnology, School of Pharmacology and Biological Sciences, University of South China, Hengyang Hunan 421001, China;Department of Laboratory Animal Science, University of South China, Hengyang Hunan 421001, China;Department of Laboratory Animal Science, University of South China, Hengyang Hunan 421001, China;Department of Laboratory Animal Science, University of South China, Hengyang Hunan 421001, China;Department of Laboratory Animal Science, University of South China, Hengyang Hunan 421001, China;Department of Biotechnology, School of Pharmacology and Biological Sciences, University of South China, Hengyang Hunan 421001, China;Department of Laboratory Animal Science, University of South China, Hengyang Hunan 421001, China |
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| Abstract: | Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell mediated autoimmune diseases. Methods We collected bone marrow-derived dentritic cells from BALB/c mice to immunize Wistar rats and to establish many hybridoma cell lines. Many hybridoma cell lines which could modulate T-cell functions were obtained. One of the cell lines, most actively inhibiting T-cell proliferation, was further studied. Results The monoclonal antibody anti-CD45 mAb recognized CD45, and the anti-CD45 mAb significantly suppressed T-cell proliferation in proliferation assays.. Conclusions Our results indicate that the anti-CD45 mAb can effectively suppress T-cell proliferation, and is promising to be used in the prevention and treatment of T-cell mediated autoimmune diseases in the future. |
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| Keywords: | CD45 Monoclonal antibody Inhibition T- cell proliferation |
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