基于1H核磁共振谱分析的自发性高血压大鼠尿液代谢特征

目的 采用¹H核磁共振谱分析结合偏最小二乘分析（PLS-DA）方法研究自发性高血压大鼠和Wistar大鼠尿液的成分谱差异，寻找可能的生物标记物。方法 选取自发性高血压大鼠和Wistar大鼠，连续4周收集尿液，以¹H-NMR分析两组大鼠的尿液成分，使用PLS-DA方法进行模式识别，通过OPLS-DA的相关系数寻找差异标记物。结果 PLS-DA方法处理高血压大鼠和Wistar大鼠的¹H-NMR谱数据显示，两组¹HNMR谱数据可以在得分图中明显区分，且代谢趋势在连续4周测定均非常稳定，说明自发性高血压大鼠有着特定的代谢模式，通过OPLS相关系数分析出体内部分氨基酸代谢产物和葡萄糖等能量代谢物质明显异于正常大鼠。结论 ¹H-NMR结合PLS-DA模式识别的代谢组学方法具有研究复杂条件下机体病理生理变化的优势，为了解高血压大鼠的代谢特征提供了科学依据.

Investigation into the urinary metabolomic profiling of spontaneously hypertensive rat (SHR) based on 1H-NMR analysis

Objective To investigate the application of metabolomics in research of the metabolic profiling of spontaneously hypertensive rats (SHR) and Wistar rats using NMR combined with PLS-DA. Methods SHRs were fed with normal diet for 1 week and then the urines of normal rats and SHR hypertensive model rats were collected at 1, 2, 3, and 4 weeks. The metabolic profiles were analyzed using ¹H-NMR. Partial least squares discriminant analysis (PLS-DA) was used to determine the differential biomarkers. Results The systolic blood pressure (SBP) of SHRs were significantly higher than that of normal rats. Using PLS-DA and orthogonal partial least squares discriminant analysis (OPLS-DA), it was also possible to distinguish them in the principal components score plot. In the SHRs, the levels of some amino acids and some small molecular substances were higher than those in the control rats. Conclusions NMR combined with PLS-DA is a promising approach to provide the information on metabolic changes related to the pathophysiological processes in spontaneously hypertensive rats.