水溶性金属卟啉的合成、表征及其清除有毒活性氧的研究

目的合成并结构表征了4种水溶性金属卟啉配合物［5，10，15，20-四［4-(4′-吡啶-1)丁氧基苯基］金属卟啉溴化盐［锌卟啉(I)、铜卟啉(II)、锰卟啉(III)和钴卟啉(IV)］，作为抗活性氧(O^-₂，H₂O₂，HO·)模拟酶。方法 核黄素-蛋氨酸光照测其清除O^-₂作用，H₂O₂氧化Vit C法测其催化H₂O₂的分解，Fenton-苯甲酸钠荧光法测其对HO·清除作用，小鼠肝匀浆法测其抗脂质过氧化作用。结果1.0×10^-5~1.0×10^-6 mol·L^-1均具有良好的清除O^-₂作用；1.5×10^-6~1.0×10^-6 mol·L^-1均具有分解H₂O₂作用；2.0×10^-8~1.0×10^-8 mol·L^-1均具有清除HO·的功能；1.0×10^-7 mol·L^-1均可使脂质过氧化产物明显减少。结论合成4种水溶性金属卟啉配合物可作为抗多种活性氧(O^-₂，H₂O₂，HO·)模拟酶。

Synthesis and anti-active oxygen properties of water-soluble metal porphyrins

AIM: To synthesize four water-soluble metal porphyrins 5, 10, 15, 20-tetra4-(4'-pyridine-1) butyloxy phenyl] metalloporphyrins bromide, metal = Zn (I), Cu (II), Mn (III) and Co (IV)] as analogous enzyme having two anti-active oxygen functions. METHODS: The first function, scavenging O2-, has been proved by using riboflavine-methionine photoreduction methods. The second function, scavenging H2O2, has been demonstrated by using the oxidating Vit C. The third function, scavenging HO*, has been demonstrated by using Fenton reaction. The complexes were measured by the mice liver homogenate technique of mice. RESULTS: Four model compounds could scavenge O2- in the concentration range of 1.0 x 10(-5) - 1.0 x 10(-6) mol x L(-1), decompose H2O2 in the concentration of 1.5 x 10(-6) - 1.0 x 10(-6) mol x L(-1), scavenge HO* in the concentration of 2.0 x 10(-8) - 1.0 x 10(-8) mol x L(-1). All showed that they had obvious action of decreasing the lipid peroxidation in the concentration of 1.0 x 10(-7) mol x L(-1). CONCLUSION: All above-mentioned complexes were considered to be qualified analogous enzymes of anti-active oxygen.