中国南海短指软珊瑚化学成分研究

目的研究短指软珊瑚Sinulariasp.的化学成分。方法利用硅胶色谱、Sephadex LH-20凝胶色谱、HPLC等手段对化学成分进行分离纯化;通过理化性质、波谱分析方法结合文献对照,鉴定化合物的结构。结果从短指软珊瑚甲醇提取物中,共分离鉴定了11个单体化合物:胆甾醇(1)、(22E)-麦角甾-5,22-二烯-3β-醇(2)、胆甾-5,20-二烯-3β-醇(3)、麦角甾-5,24(28)-二烯-3β-醇(4)、柳珊瑚甾醇(5)、3β-羟基胆甾-5-烯-7-酮(6)、3β-羟基麦角甾-5,24(28)-二烯-7-酮(7)、(22E)-3β-羟基麦角甾-5,22-二烯-7-酮(8)、3β-羟基麦角甾-5-烯-7-酮(9)、(22E)-3β-羟基胆甾-5,22-二烯-7-酮(10)、鲨肝醇(11)。结论化合物6～10为首次从该属软珊瑚中分离得到。化合物7在10μg.mL-1浓度水平,对K562肿瘤细胞株的抑制率为22.74%,对HeLa肿瘤细胞株的抑制率为9.98%。

Studies on chemical constituents of Sinularia sp.

Objective To study the constituents of Sinularia sp.Methods The isolation and purification of the compounds were performed by silica gel,Sephadex LH-20 and HPLC methods,and their structures were determined by comparison their physicochemical characters and spectral data with literatures.Results From the methanol extracts of Sinularia sp.,eleven compounds were isolated and indentified as cholesterol(1)、(22E)-ergosta-5,22-dien-3β-ol(2)、cholesta-5,20-dien-3β-ol(3)、ergosta-5,24(28)-dien-3β-ol(4)、gorgosterol(5)、3β-hydroxycholesta-5-en-7-one(6)、3β-hydroxyergosta-5,24(28)-dien-7-one(7)、(22E)-3β-hydroxyergosta-5,22-dien-7-one(8)、3β-hydroxyergosta-5-en-7-one(9)、(22E)-3β-hydroxycholesta-5,22-dien-7-one(10) and batyl alcohol(11),respectively.Conclusion Compounds 6~10 were isolated from this soft coral for the first time,and compound 7 exhibited weak cytotoxicity against the K562 and HeLa cell line with the inhibition rate 22.74% and 9.98% at the concentration level of 10 μg·mL-1,respectively.