五参二连颗粒对病毒性心肌炎小鼠免疫调节机制的研究

目的:研究五参二连颗粒对病毒性心肌炎(VMC)小鼠心肌保护作用及免疫调节机制的研究。方法:用柯萨奇B3型病毒(CoxB3)建立BALB/c小鼠VMC模型。将60只BALB/c小鼠随机分为正常组、模型组、阳性药物利巴韦林组(1 mg·kg-1)及五参二连低、中、高剂量组(4,12,20 mg·kg-1)。造模后第0,1,2,3,4,5天,用药组分别给与相应药物灌胃给药并观察动物一般情况。连续给药5 d,在末次给药2 h后终止实验,称重、并根据检测要求做标本采集。对所取标本进行心肌酶指标检测、脏器指数检测及自然杀伤细胞(NK)杀伤实验。结果:模型组小鼠第2天后开始精神萎靡,活动减少,毛粗糙,体温下降。第5天后处死,心脏晦暗,纹理不清,心脏表面出现白色点状条索状病变。病理学HE染色,心肌组织有大量的炎性细胞浸润,可广泛弥漫,也可呈局灶性,并可有坏死灶。同时模型组的肌酸激酶同工酶、肌钙蛋白、肌红蛋白与正常对照组比较P<0.05,表明造模成功。利巴韦林组,五参二连颗粒高、中剂量组的肌酸激酶同工酶、肌钙蛋白均有下降,与模型对照组比较P<0.05。利巴韦林组及五参二连颗粒高、中剂量组脾指数和胸腺指数有升高,与模型对照组比较P<0.05。利巴韦林及五参二连颗粒高、中剂量组NK杀伤作用与正常对照组和模型对照组比较P<0.05。结论:五参二连颗粒可以减轻CoxB3造成的心肌损伤,并激发机体的免疫调节机制,对心肌细胞有一定保护作用。

Immune Regulation Mechanism of Wushen Erlian Granules in Mice with Viral Myocarditis

Objective: To study the mechanism of protection and immune regulation of Wushen Erlian granules in mice with viral myocarditis myocardial. Method: BALB/c mice with viral myocarditis model was established by Coxsackie B3 virus(CoxB3 ).The BALB/c mices were randomized into the normal group,model group, positive control ribavirin group(1 mg·kg^-1)and Wushen Erlian granule low-dose group, middle-dose group, high-dose group (4,12,20 mg·kg^-1). After modeling, corresponding drugs were respectively given to each mice in all groups at d0, d1,d2,d3,d4,d5 for 5 days. Weighing and specimen collection according to the testing requirements were carried out. Myocardial enzyme indexes detection, viscera index detection and natural killer cells(NK)killing experiment were performed. Result: After second days, micein model group appeared apathetic, reduced action, rough, hypothermia. After fifth days, mice were killed and the heart was dark, texture was not clear, the heart surface appeared white spot funicular lesions. Pathological HE staining indicated inflammatory cell and necrotic lesionsof myocardial tissue. At the same time, Compared with normal group, creatine kinase isoenzyme, troponin and myoglobin of model animals were increased significantly (P<0.05), which showed the model was effective. Compared with model group, creatine kinase isoenzyme and troponin in ribavirin group, high dose group and middle dose group of Wushen Erlian granule were declined, with statistically significant difference(P<0.05). Spleen index and thymus index in ribavirin group, high dose group and middle dose group of Wushen Erlian granule were increased (P<0.05). The test result of killing effect of NK showed ribavirin, high dose and middle dose of Wushen Erlian granule group compared with model group and normal group were significant different(P<0.05). Conclusion: Wushen Erlian granules can alleviate myocardial injury caused by CoxB₃ and stimulate immune regulation mechanism of the body, with has certain protective effect on myocardial cells.