| 小果鹧鸪花果实脂溶性成分GC-MS分析及抗菌活性 |
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| 引用本文： | 刘寿柏,梅文莉,郭志凯,戴好富.小果鹧鸪花果实脂溶性成分GC-MS分析及抗菌活性[J].中国实验方剂学杂志,2014,20(19):105-108. |
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| 作者姓名： | 刘寿柏 梅文莉 郭志凯 戴好富 |
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| 作者单位： | 海南大学园艺园林学院, 海口 570228;中国热带农业科学院热带生物技术研究所/海南省黎药资源天然产物研究与利用重点实验室, 海口 571101;中国热带农业科学院热带生物技术研究所/海南省黎药资源天然产物研究与利用重点实验室, 海口 571101;海口市热带天然产物研究与利用重点实验室, 海口 571101;中国热带农业科学院热带生物技术研究所/海南省黎药资源天然产物研究与利用重点实验室, 海口 571101;海口市热带天然产物研究与利用重点实验室, 海口 571101;中国热带农业科学院热带生物技术研究所/海南省黎药资源天然产物研究与利用重点实验室, 海口 571101;海口市热带天然产物研究与利用重点实验室, 海口 571101 |
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| 基金项目： | 国家科技支撑计划项目（2013BAI11B04）；公益性行业（农业）科研专项（201303117） |
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| 摘 要： | 目的:为研究小果鹧鸪花Trichilia connaroides Bentv.var.microcarpa Bentv.果实的脂溶性成分及抗菌活性。方法:冷浸法提取小果鹧鸪花果实中脂溶性成分,采用气相色谱-质谱联用技术(GC-MS)对其化学成分进行分析;用滤纸片琼脂扩散法测定其抗菌活性。结果:共分离出45个色谱峰,鉴定了其中44个化合物,占总量的92.82%;该脂溶性成分对金黄色葡萄球菌具有抑制作用。结论:首次从小果鹧鸪花果实中提取脂溶性成分并鉴定化学成分,主要为脂肪酸类化合物(60.88%),为小果鹧鸪花的开发利用提供了科学依据。
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| 关 键 词： | 小果鹧鸪花 脂溶性成分 气相色谱-质谱联用 抗菌活性 |
| 收稿时间： | 2013/12/13 0:00:00 |
GC-MS Analysis and Antibacterial Activity of Liposoluble Components from Fruit of Trichilia connaroides var. microcarpa |
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| LIU Shou-bai,MEI Wen-li,GUO Zhi-kai and DAI Hao-fu.GC-MS Analysis and Antibacterial Activity of Liposoluble Components from Fruit of Trichilia connaroides var. microcarpa[J].China Journal of Experimental Traditional Medical Formulae,2014,20(19):105-108. |
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| Authors: | LIU Shou-bai MEI Wen-li GUO Zhi-kai and DAI Hao-fu |
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| Institution: | Horticultural and Garden College, Hainan University, Haikou 570228, China;Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;Haikou Key Laboratory for Research and Development of Tropical Natural Products, Haikou 571101, China;Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;Haikou Key Laboratory for Research and Development of Tropical Natural Products, Haikou 571101, China;Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;Haikou Key Laboratory for Research and Development of Tropical Natural Products, Haikou 571101, China |
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| Abstract: | Objective: To study chemical constituents andantibacterial activity of the liposoluble extract from the fruit of Trichilia connaroides var. microcarpa. Method: The liposoluble components was extracted by cold-soak extraction, and their componentswas analyzed by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was assayed by paper disk diffusion method. Result: Forty-five peaks were separated and forty-four of them were identified, representing about 92.82% of the total content. The liposoluble extract of the Fruit of T. connaroides var. microcarpa exhibited inhibitoryeffect on Staphylococcus aureus. Conclusion: The liposoluble componentswere extracted for the first timeandthe compoundswere identified. The fatty acids were the main components, accounting for 60.88% of the total liposoluble components. This research could providea scientific basis for further research and development on the fruit of T. connaroides var. microcarpa. |
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| Keywords: | Trichilia connaroides var microcarpa liposoluble components GC-MS antibacterial activity |
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