锦红片对胆管炎大鼠胸腺的影响

目的:探讨急性胆管炎状态下细胞免疫中枢胸腺的变化以及中药锦红片的干预作用。方法:雄性清洁级SD大鼠24只,随机分为3组,每组8只。胆总管单纯结扎组(简称单纯结扎组):结扎胆总管,但不注射细菌,手术后喂生理盐水;模型组:建立大鼠急性胆管炎模型,造模手术后喂生理盐水;锦红片治疗组:造模手术后喂锦红片悬液。手术后5d处死大鼠,胸腺取材,做胸腺电镜和光镜形态学观察,并检测胸腺质量、胸腺指数、凋亡指数、胸腺Bcl-2基因编码蛋白表达和胸腺Fas基因编码蛋白的表达。结果:形态学上凋亡细胞出现频率由高到低依次为模型组、锦红片治疗组和单纯结扎组。模型组胸腺质量和胸腺指数均降低,与锦红片治疗组和单纯结扎组相比,差异有统计学意义。模型组胸腺凋亡指数明显高于锦红片治疗组和单纯结扎组。锦红片治疗组Bcl-2基因编码蛋白表达高于模型组,Fas基因编码蛋白表达组间差异无统计学意义。结论:急性胆管炎时大鼠胸腺质量、胸腺指数降低,胸腺凋亡指数升高,非生理性凋亡增加。清热通下中药锦红片有一定的抑制这种非生理性凋亡的作用,这种作用可能是通过上调凋亡抑制基因Bcl-2编码蛋白的表达实现的。

Impact of Jinhong Tablet on thymus of rats with cholangitis

Objective: To study the changes of the center of cellular immune thymus and the interference of Jinhong Tablet, a compound traditional Chinese herbal medicine, during the course of cholangitis. Methods: Twenty-four male Wistar rats were randomly divided into three groups. Ligation group, simple ligation of bile duct without producing infection and fed with normal saline; untreated group, reproduce acute cholangitis and fed with normal saline; Jinhong Tablet-treated group, reproduce cholangitis and treated with Jinhong Tablet. Five days after modeling, the rats were sacrificed and the thymus were sampled to observe the morphological changes with an electronic microscope, count the index of thymus and apoptosis and assay the expressions of Bcl-2 gene coding protein and Fas gene coding protein. Results: The frequency of apoptosis of thymus in the untreated group was higher than that in the Jinhong Tablet-treated group and the ligation group, and the frequency of apoptosis of thymus in the Jinhong Tablet-treated group was higher than that in the ligation group. Compared with the Jinhong Tablet-treated group and the ligation group, the weight and index of thymus were lower and the index of apoptosis of thymus was higher significantly in the untreated group (P<0.01). The expression of Bcl-2 gene coding protein in the ligation group and the Jinhong Tablet-treated group were much higher than that in the untreated group (P<0.01), but there were no significant differences of the expression of Fas gene coding protein among the three groups. Conclusion: There exists a promotion of non-physiological apoptosis of cellular immune center such as decrease of the weight and the index of thymus and increase of the index of apoptosis of thymus during acute cholangitis, which may be inhibited by up-regulation of Bcl-2 gene coding protein with antipyretic and purgative herbs Jinhong Tablet.