基于网络药理学探讨白藜芦醇抗衰老的作用机制

目的:运用网络药理学方法探寻白藜芦醇抗衰老的关键靶点及分子作用机制。方法:通过PharmMapper数据库查找白藜芦醇的作用靶点;采用GeneCards数据库搜索衰老的相关蛋白。将白藜芦醇作用靶点和疾病靶点取交集,即为白藜芦醇抗衰老潜在靶点。采用STRING数据库找出靶点的作用关系,以DAVID数据库分析结果,Cytoscape3.8.0软件构建药物-疾病-靶点-通路网络。结果:从PharmMapper得到的91个白藜芦醇(Resveratrol,Res)作用靶点,GeneCards数据库得到的474个衰老相关靶点,两者取交集后共得到22个白藜芦醇抗衰老的作用靶点。在蛋白相互作用网络中IGF1、ESR1、MAPK1和EGFR处于核心位置。在GO富集分析和KEGG通路分析中,EGFR、MAPK1和IGFR均存在于PI3K-Akt通路、FoxO通路和HIF-1通路中。在药物-疾病-靶点-通路网络中,EGFR和MAPK1处于核心位置。结论:Res通过EGFR、MAPK1、IGFR调节PI3K-Akt和FoxO通路抗衰老。

Anti-aging mechanism of resveratrol through the assays of network pharmacology approach

Objective:To explore the molecular anti-aging mechanism of resveratrol through network pharmacology approach.Methods:All targets were imported into the STRING database,DAVID database and Cytoscape3.8.0 to construct a“component-disease-target”interaction network.Results:A total of 91 targets and 474 aging-related targets obtained from PharmMapper and GeneCards database.Twenty-two targets for resveratrol anti-aging action were obtained.IGF1,ESR1,MAPK1 and EGFR are at the core of the protein interaction network.EGFR,MAPK1 and IGFR are all found in the PI3K-Akt pathway,FoxO pathway and HIF-1channel in GO enrichment analysis and KEGG path analysis.EGFR and MAPK1 are at the core of the“component-disease-target”network.Conclusion:Resveratrol regulates PI3K-Akt pathway and FoxO pathway to anti-aging through EGFR,MAPK1 and IFR.