EphA2mRNA和VEGF在肝癌细胞中的表达及其关系

目的探讨肝癌（hepatocellular carcinoma，HCC）细胞中EphA2 mRNA（erythropoietin producing hepatoma cell lines）和VEGF（vascular endothelial growth factor）的表达及其关系。方法用原位杂交的方法检测53例HCC组织及其癌旁组织中EphA2 mRNA表达，并用免疫组织化学的方法检测HCC组织中VEGF的表达。结果53例肝细胞癌组织及癌旁组织中EphA2 mRNA的阳性率分别为86．79％（46／53）和69．81％（37／53），其差异有显著的统计学意义（W=2289．500，P〈0．001）；肝癌组织中VEGF的阳性率为75．47％（40／53），癌旁组织中VEGF的表达为阴性。肝癌组织中EphA2 mRNA和VEGF的表达显示：（1）表达水平与是否患有肝硬化无关；（2）表达水平分别与肝癌的临床病理学Edmondson分级呈明显的正相关（r=0．452，P=0．001；r=0．333，P=0．015）；（3）二者之间的表达水平呈明显的正相关（r=0．425，P=0．002）。结论本实验表明：肝癌细胞中EphA2 mRNA和VEGF呈过量表达；肝癌细胞中EphA2 mRNA和VEGF的表达与肝癌细胞恶性程度以及二者之间密切关联。

Expression of EphA2 mRNA and VEGF in human hepatocellular carcinoma and their relationship

Objective To clarify the expression of EphA2mRNA and VEGF in hepatocellular carcinoma (HCC) and explore their relationship. Methods The expression of EphA2mRNA was determined with in situ hybridization and that of VEGF with immunohistochemistry in cancerous and adjacent tissues from 53 cases of HCC. Results The rate of expression of EphA2mRNA was significantly higher in HCC tissue than in the adjacent tissue (86. 79% vs. 69. 81%, P<0. 001). The rate of expression of VEGF in HCC tissue was 75. 4% (40/53) and there was no expression of VEGF in the adjacent tissue. The expression of EphA2mRNA and VEGF in HCC was markedly correlated with tumor cell grade (P<0. 01 and 0. 05). EphA2mRNA level was positively correlated with VEGF level (P<0. 01). Conclusions EphA2mRNA and VEGF are over-expressed in HCC and the over-expression is closely related to the malignant behaviors of the tumor. Furthermore, there is a significant relationship between the expression of EphA2mRNA and that of VEGF in HCC.