6-羟基多巴脑内注射建立帕金森病大鼠模型的实验研究

目的 探讨6-羟基多巴脑内注射建立稳定的帕金森病大鼠模型的方法.方法 将6-羟基多巴立体定向注入大鼠右侧前脑内侧束和中脑被盖腹侧区,观察大鼠的行为变化和中脑黑质区的形态学变化.结果 注药后2周,动物经阿扑吗啡诱导即出现向健侧的旋转行为,注射效果稳定.在长达3个月的观察中,动物的旋转行为稳定,无明显的差异.形态学显示,毁损侧的黑质致密部和中脑被盖腰侧区的酪氨酸羟化酶阳性神经元的数量明显减少.结论 通过6-羟基多巴脑内注射的方法可以建立起稳定、有效的帕金森病大鼠模型.

Experimental Study on Establishment of Rats Model of Parkinson Disease by 6-OHDA Injection

Objective To study the method of establish steady animal model of PD by 6-OHDA injection.Methods Rats received a unilateral microinjection of 6-hydroxydopamine into the right ventral tegmental area(VTA) and the right median forebrain bundle(MFB).After lesion surgery,animals were tested for the Apomorphine-induced rotational behavior every week.Three months later,the changes of tyrosine hydroxylase-like neurons in the SN were investigated with immunocytochemical methods.Results Two weeks after the injection,rats exhibited contra lateral rotation to the lesion side.During the following months,the rotation behavior was steady.The number of TH-immunoreactive(TH-ir) cells in the Substantia nigra pars compacta(SNc)and VTA on the injected side decreased dramatically.Conclusion Unilateral interval injection of 6-OHDA can establish PD rat models steadily and effectively.