| 幽门螺杆菌尿素酶A、过氧化氢酶的表达及纯化 |
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| 引用本文： | 廖文俊,陈湖,朱森林,陈洁,陈为,胡品津.幽门螺杆菌尿素酶A、过氧化氢酶的表达及纯化[J].中国免疫学杂志,2001,17(7):348-350. |
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| 作者姓名： | 廖文俊 陈湖 朱森林 陈洁 陈为 胡品津 |
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| 作者单位： | 1. 第四军医大学附属西京医院皮肤科,
2. 中山医科大学附属第一医院消化内科, |
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| 基金项目： | 卫生部临床学科重点项目(No.97040226)、广东省科技计划重点攻关项目(No.99No4802G)和广东省自然科学基金课题(No.990077) |
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| 摘 要： | 目的：探讨重组尿素酶A亚单位（UreA）、过氧化氢酶（KatA）疫苗在防治婴门螺杆菌（HP）感染中的作用。方法：构建表达UreA、KatA的重组质粒，用IPTG诱导表达融合蛋白，并进行SDS－PAGE凝胶电泳及Western blot分析，Bulk GST Purification Module试剂盒纯化UreA和KatA。结果：构建的重组质粒能表达GST－UreA和GST－KatA合蛋白，表达量占细胞总蛋白量的35％和19％，并能与抗GST 发生特异性反应。纯化的UreA、KatA的纯度达95％以上。结论：该工作为进一步的动物免疫实验奠定了基础，该重组疫苗有望在HP感染及其相关疾病的防治中发挥积极作用。
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| 关 键 词： | 幽门螺杆菌 疫苗 尿素酶A 过氧化氢酶 |
| 文章编号： | 1000-484X(2001)07-0348-03 |
Expression and purification of Helicobacter pylori urease A subunit and catalase |
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| LIAO Wen Jun,CHEN Min Hu,ZHU Sen Lin et al.Expression and purification of Helicobacter pylori urease A subunit and catalase[J].Chinese Journal of Immunology,2001,17(7):348-350. |
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| Authors: | LIAO Wen Jun CHEN Min Hu ZHU Sen Lin |
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| Institution: | LIAO Wen Jun,CHEN Min Hu,ZHU Sen Lin et al Department of Gastroenterology,The First Affiliated Hospital,Sun Yat Sen University of Medical Sciences,Guangzhou 510080 |
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| Abstract: | Objective:Investigated the effect of recombinant urease A subunit and catalase vaccines on protection against Helicobacter pylori (HP) infection Methods:Recombinant plasmids expressing UreA or KatA were constructed Expression was induced with IPTG,analyzed by SDS PAGE and Western blot UreA and KatA were purified with Bulk GST purification module Results:The recombinant plasmids could express GST UreA and GST KatA fusion proteins,which accounted for 35% and 19% of total bacteria proteins respectively,and could specially react with antibody against GST The purity of the purified UreA and KatA was over 95% Conclusion:The work laid a foundation for further studies of HP vaccine The recombinant vaccines may play an important role in preventing and treating HP infection and related diseases |
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| Keywords: | Helicobacter pylori Vaccine KatA Urease |
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