肾康灵对肾大部切除大鼠肾脏的保护作用

目的：观察肾康灵在5／6肾大部切除大鼠模型中对残肾的保护作用并探讨其可能的机制。方法：将50只大鼠随机分为5组：假手术组、模型组、苯那普利治疗组（10nag·kg^-1·d^-1）、肾康灵高剂量组（15g·kg^-1·d^-1）和肾康灵低剂量组（5g·kg^-1·d^-1）。8周后观察大鼠24h尿蛋白、BUN、Scr、Alb及肾脏病理改变，并用免疫组化法观察转化生长因子pI（TGF—β1）、Col—Ⅳ、纤连蛋白（FN）、层黏连蛋白（LN）的表达。结果：肾康灵高低剂量治疗组和苯那普利治疗组尿蛋白排出量、肌酐、尿素氮水平明显低于模型组，血浆白蛋白水平高于模型组（P〈0．05）。病理上，接受肾大部切除术的大鼠均可见基质增生、肾小球硬化，但与模型组相比，用药组肾小球硬化程度明显减轻，其中肾康灵高剂量组肾小球硬化程度最轻；免疫组化结果显示用药组TGF-β1均明显低于模型组（P〈0．01），且肾康灵高、低刺量组与苯那普利组之间比较无统计学意义（P〉0．05）。结论：肾康灵对5／6肾大部切除大鼠肾脏有明显保护作用，其机制可能与其能减少肾组织中TGF-β1的表达、减少细胞外基质、降低蛋白尿等有关。

Nephroprotective Effects of Shenkangling on Rat Remnant Kidney

Objective-To investigate the nephroprotective effects and mechanism of Shenkangling on 5/6 nephrectomized rats. Methods： 50 wistar rats were randomly divided into 5 groups： Sham operation group ,5/6 nephrectomized group, Lotensin group （ 10 mg· kg^-1· d^-1 ） ,ShenKangling high dosage group（ 15 mg· kg^-1· d^-1） and Shenkangling low dosage group（ 5 g· kg^-1· d^-1 ）. Urinary protein,BUN,Scr and ALB were measured after 8 weeks at the end of the study. Glomemlosclerosis index were evaluated by PAS and HE stain. Immunohistochemical method was used to examine the expression of fibrnectin（FN） ,laminin（ LN）, collagen IV and transforming grouth factor β1 （TGF- β1 ）. Results： Compared with the untreated 5/6 nephrectomized group, urinary protein, BUN and SCr of ShenKangling group and Lotensin group were substantially decreased, while ALB were increased（ P 〈 0.05 ）. Pathohistological study revealed that compared with the untreated 5/6 nephrectomized group, there were less extracellular matrix, typeⅣ collagen,fibronectin and laminin in ShenKangling and Lotensin groups. Among the 5/6 nephrecrotnized rats, ShenKanglin highdosage treated group had the least renal lesions in terms of glomerulcsclerosis; immunohistochemical result revealed that compared with the untreated 5/6 nephrectomized group, there were less TGF - 131 in ShenKangling and Lotensin groups, and there were no significant difference between ShenKanglin and Lotensin groups（ P 〉0.05 ）. Conclusion： Shenkangling can prevent renal injury following subtotal renal ablation which might be linked with its decreasing the level of TGF- β1 in the kidney of 5/6 nephrectomized rats.