| 高效液相色谱法测定注射用匹多莫德中匹多莫德的含量 |
| |
| 引用本文： | 刘宏,茆玲. 高效液相色谱法测定注射用匹多莫德中匹多莫德的含量[J]. 中国医药导报, 2010, 7(10): 88-89 |
| |
| 作者姓名： | 刘宏 茆玲 |
| |
| 作者单位： | 1. 吉林省通化市人民医院药剂科,吉林,通化,134001
2. 南京市六合区人民医院药剂科,江苏,南京,211500 |
| |
| 摘 要： | 目的：建立高效液相法测定注射用匹多莫德中匹多莫德含量的方法。方法：采用Diamonsil-C18（4.6mm×250mm,5μm）色谱柱;流动相：0.01mol/LNaH2PO4-甲醇-水（97∶2∶1）（磷酸调节pH至pH=2.5）;检测波长：210nm;流速：1.0ml/min。结果：匹多莫德在0.2732～4.3712μg范围内与峰面积呈良好的线性关系（r=0.9997）,平均回收率为99.66%,RSD=0.80%（n=6）。结论：本方法简便、准确、重现性好,可用于注射用匹多莫德中匹多莫德的质量控制。
|
| 关 键 词： | 高效液相法 匹多莫德 注射用匹多莫德 含量测定 |
Content determination of pidotimod and its related substances in Pidotimod for Injection by HPLC |
| |
| LIU hong,MAO Ling. Content determination of pidotimod and its related substances in Pidotimod for Injection by HPLC[J]. China Medical Herald, 2010, 7(10): 88-89 |
| |
| Authors: | LIU hong MAO Ling |
| |
| Affiliation: | 1.Department of Pharmaceutics,the People＇s Hospital of Tonghua,Tonghua 134001,China; 2. Department of Pharma-ceutics,the People＇s Hospital of Nanjing,Nanjing 211500,China) |
| |
| Abstract: | Objective：To establish a method for the content determination of pidotimod in Pidotimod for Injection by HPLC. Methods：The Diamonsil-C18 （4.6 mm×250 mm,5 μm） column was used. The mobile phases consisted of 0.01 mol/L-sodium dihydrogen phosphate dihydrate,methanol and water （97∶2∶1） （adjust pH to pH=2.5 by phosphoric acid）,the flow rate was 1.0 ml/min and the detection wavelength was at 210 nm. Results：The linear ranges of pidotimod was at 0.273 2-4.371 2 μg （r=0.999 7）,the average recovery was 99.66% with a RSD of 0.80% （n=6）. Conclusion：The method is fast,reliable,accurate and can be used in the quality control of pidotimod in Pidotimod for Injection. |
| |
| Keywords: | HPLC Pidotimod Pidotimod for Injection Content determination |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
