巨核细胞计数方法对其分类结果影响的研究论著

目的研究巨核细胞计数方法对其分类结果的影响,为制定巨核细胞的正常值提供前提。方法计数骨髓穿刺细胞学检查无明显异常者262例对象骨髓涂片巨核细胞总数,选取单位面积内巨核细胞数目大于100者,研究计数25个、50个巨核细胞与计数100个对其分类的影响;采用"弓"及"W"字形依次寻找巨核细胞,比较其对巨核细胞分类的影响。结果 262例研究对象骨髓涂片中巨核细胞总数范围为9～157个/1.5 cm×3.0 cm,平均(48.4±32.2)个。计数50个巨核细胞与计数100个比较,对巨核细胞分类的影响没有统计学意义;低倍镜下采用"弓"及"W"字形依次计数方法对巨核细胞分类的影响没有统计学差异。但是采用"W"字形寻找巨核细胞的方法幼巨核细胞数目偏高,产血小板型巨核细胞数目偏低。结论建议采用分类50个巨核细胞、"弓"字形寻找巨核细胞的方法。

Effect of the megakaryocyte count method for its classification results

Objective To study the effect of the megakaryocyte count method for its classification results. This method can serve as the basis for its normal range. Methods The total number of megakaryocytes of bone marrow aspiration in 262 cases were counted. The number of megakaryocytes per unit area greater than 100 was included into this study. We compared the influence and classificaiton of megakaryocyte at 25, 50 and 100 per unit. The megakaryocyte were found according the ＂ Bow＂ and ＂ W＂ shape. The impact on the classification of megakaryocytes were compared. Results The average number of megakaryocytes in the 262 cases is 48.4 ＋ 32.2. There was not statistically significant impact on the classification of megakaryocyte in counting of 50 and 100. But there was more promegakaryocytes and less platelet megakaryocyte through ＂W＂ shape. Conclusion Recommended classification 50 megakaryocyte, the megakaryocyte were found according the ＂Bow＂ shape.