| 透明质酸钠的光谱学分析 |
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| 引用本文： | 樊东辉,吴蓓蓓,徐政,顾其胜.透明质酸钠的光谱学分析[J].中国生化药物杂志,2006,27(1):22-25. |
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| 作者姓名： | 樊东辉 吴蓓蓓 徐政 顾其胜 |
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| 作者单位： | 1. 同济大学,材料学院,上海,200092
2. 同济大学,材料学院,上海,200092;上海其胜生物材料技术研究所,上海,201106 |
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| 摘 要： | 目的对透明质酸钠(HA)的结构进行表征。方法用红外光谱法测定HA的一级结构,采用拉曼光谱法作为佐证。在认识HA中存在的化学基因后,用圆二色谱测定HA的三级结构进行探索。结果在此实验条件下所得到的红外光谱与美国材料实验协会提出的谱图基本上吻合,同时出现新的吸收峰。拉曼光谱的吸收峰位置与红外光谱相一致,并且观测到低于500 cm-1的波数有吸收峰出现。HA的圆二色谱图在210 nm有特征性的负峰出现。结论HA分子结构中存在醇类羟基,并且羟基之间发生了很大程度的缔合,圆二色谱图中的负峰表示HA在溶液中的形态为β构象,这些是实现HA黏弹性的基础。
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| 关 键 词： | 透明质酸钠 拉曼光谱 红外光谱 圆二色谱 |
| 文章编号： | 1005-1678（2006）01-0022-04 |
| 收稿时间： | 2005-04-06 |
| 修稿时间： | 2005-06-28 |
Analysis of hyaluronan by spectroscopy |
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| FAN Dong-hui,WU Bei-bei,XU Zheng,GU Qi-sheng.Analysis of hyaluronan by spectroscopy[J].Chinese Journal of Biochemical Pharmaceutics,2006,27(1):22-25. |
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| Authors: | FAN Dong-hui WU Bei-bei XU Zheng GU Qi-sheng |
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| Institution: | 1. School of Material Science and Technology, Tongji University, Shanghai 200092, China; 2. Shanghai Qisheng Biological Material Research Institute, Shanghai 201106, China |
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| Abstract: | PurposeTo characterize the structure of hyaluronan(HA).MethodsInfrared spectrum was adopted to measure the first structure of HA,with the results of Raman spectrum as a proof.On the basis of knowledge of the groups in HA,circular dichroism was taken to explore the tertiary structure of HA.ResultsThe Infrared spectrum acquired under the condition of this essay mostly accords with that issued by ASTM,with some minor difference.The absorption peaks in the Raman spectrum of HA correspond to those in the Infrared spectrum,and some peaks may be observed below 500 cm~(-1).A characteristic negative peak appears in the circular dichroism of HA.ConclusionMellow hydroxyls exist in the hyaluronan molecule,with a large degree of association,and hyaluronan takes the form of β-folding in solution,which contributes to the viscosity of HA. |
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| Keywords: | hyaluronan Raman spectrum FT-IR spectrum circular dichroism |
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