怀槐异黄酮水解后苷元的含量测定

目的用HPLC法测定怀槐异黄酮水解后苷元芒柄花黄素、阿夫罗摩辛、黄豆黄素的含量。方法经正交试验确定的最佳提取与水解工艺制备供试品溶液,采用高效液相法同时测定水解后3种异黄酮苷元含量。色谱柱:DiamonsilTMC18(250 mm×4.6 mm,5μm),流动相:乙腈-质量分数为0.05%的磷酸水溶液(体积比为35∶65),流速:1.0 mL.min-1,检测波长:260 nm,柱温:35℃。结果芒柄花黄素在5.0~25.2 mg.L-1、阿夫罗摩辛在4.2~21.0 mg.L-1、黄豆黄素在4.8~24.0 mg.L-1时峰面积与质量浓度呈良好的线性关系,相关系数分别为1.000 0、0.999 6、0.999 6。芒柄花黄素、阿夫罗摩辛、黄豆黄素的平均回收率分别为98.43%、97.62%、98.38%,RSD分别为1.04%、0.52%、0.23%。结论HPLC测定方法可用于芒柄花黄素、阿夫罗摩辛、黄豆黄素的含量测定。

Determination of the contents of isoflavone glycosides from hydrolyzate of Maackia amurensis

Objective To determine the contents of formononetin, afromosin and glycitein after hydrolysis from the bark of Maackia amurensis by HPLC. Methods A Diamonsil^TM C₁₈ column was used for HPLC; the mobile phase was acetonitrile and aqua containing 0.05%（w）phosphoric acid with the volume ratio of 35:65 and the UV detection wavelength was set at 260 nm; the flow rate was 1.0 mL•min^-1; the column temperature was 35 °C. Results The linear range of formononetin was 5.04-25.2 mg•L^-1(r=1.000 0), the average recovery was 98.43% with RSD of 1.04; the linear range of afromosin was 4.2-21.0 mg•L^-1 (r=0.999 6), the average recovery was 97.62% with RSD of 0.52, the linear range of glycitein was 4.8-24 mg•L^-1 (r=0.999 6), the average recovery was 98.38% with RSD of 0.23%.Conclusions The method is stable, precise, reproductive and can be applied to the determination of formononetin, afromosin and glycitein .