| 不同剂量辐射损伤后T淋巴细胞亚群,TH1和TH2变化观察 |
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| 引用本文： | 郑辉,涂序珉,甄荣,张志兴. 不同剂量辐射损伤后T淋巴细胞亚群,TH1和TH2变化观察[J]. 中国辐射卫生, 2009, 18(1): 13-15. DOI: 10.13491/j.cnki.issn.1004-714x.2009.01.055 |
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| 作者姓名： | 郑辉 涂序珉 甄荣 张志兴 |
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| 作者单位： | 中国疾病预防控制中心辐射防护与核安全医学所, 北京 100088 |
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| 摘 要： | 目的 观察不同剂量辐射损伤对小鼠T淋巴细胞功能亚群的影响,探讨辐射所致的免疫系统损伤在细胞学和分子水平上的机制。方法 C57BL/6j小鼠采用60Coγ射线照射诱导辐射损伤模型。总照射剂量分别为0.7、1.4、2.8、和5.6Gy。应用细胞表面标志和细胞内因子标记,流式细胞仪检测分析不同剂量组小鼠脾T淋巴细胞功能亚群CD3,CD4,CD8改变及Th1和Th2的变化。结果 1.小鼠脾T-淋巴细胞功能亚群CD3,CD4和CD8在照射后均有明显降低,降低幅度与受照剂量明显相关。2.CD4/CD8比值显示照射后均有明显升高。3.照射后Th1和Th2均有明显降低,其中Th1降低明显高于Th2。受照剂量大于2.8Gy组Th2/Th1比值较空白对照组明显升高。结论 不同照射剂量对T淋巴细胞功能亚群CD3,CD4,CD8和CD4/CD8有明显影响,Th1和Th2和Th2/Th1功能亚群的失衡在辐射所致的免疫损伤中具有较重要作用。
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| 关 键 词： | 辐射免疫损伤 T淋巴细胞亚群 淋巴细胞因子 Th1 Th2 |
| 收稿时间： | 2008-07-14 |
Observation the Changs of T- Lymphocyte Subsets CD3 ,CD4,CD8 ,Th1 and Th2 After Exposure to Various Dose of Irradiation |
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| ZHENG Hui,TU Xu-min,ZHEN Rong,et al.. Observation the Changs of T- Lymphocyte Subsets CD3 ,CD4,CD8 ,Th1 and Th2 After Exposure to Various Dose of Irradiation[J]. Chinese Journal of Radiological Health, 2009, 18(1): 13-15. DOI: 10.13491/j.cnki.issn.1004-714x.2009.01.055 |
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| Authors: | ZHENG Hui TU Xu-min ZHEN Rong et al. |
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| Affiliation: | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088 China |
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| Abstract: | Objective To observe the effects of T-lymphocyte subsets CD3,CD4,CD8 and CD4/CD8 after different dose of irradiation and investigate the cellular and molecular mechanism of immune system injury induced by irradiation.Methods The C57BL/6j mice were divided into normal control and model groups.The mice in radiation hurts model were induced by 60Co gamma rays(0.7,1.4,2.8 and 5.6Gy).The surface marker and intracellular cytokines of lymphocytes were stained with fluorescence labeled monoclonal antibodies.The cha... |
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| Keywords: | Radiation Immune Harm T-lymphocyte Subsets Th1 Th2 |
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