康复训练对脑梗死大鼠大脑皮质一氧化氮合酶阳性神经元表达的影响

目的：研究康复训练对脑梗死大鼠大脑皮质中一氧化氮合酶（NOS）阳性神经元表达的影响。方法：光化学法诱导大鼠脑梗死24h后，将大鼠随机分为康复训练组和制动组，康复训练组每天进行水迷宫、转棒、滚笼训练，制动组网状笼内固定。在1、2、3、4周时观察大脑皮质NOS阳性神经元的表达。结果：正常大鼠大脑皮质中即有NOS阳性神经元表达，梗死后24h梗死灶周围及对侧皮质中NOS阳性神经元表达明显增多；1．2周时梗死灶周围皮质中NOS阳性神经元表达减少，制动组较康复训练组明显，3、4周时两组相差不显著。结论：NO合成和释放增多在脑缺血所致的脑损伤早期中起着重要作用；康复训练可促进脑损伤周围及对侧神经组织修复和代偿。

Effects of rehabilitation training on the expression of NOS-reactive neurom in the cortex of cerebral infarcted

Objective To study the effect of rehabilitation training on the cxpression of NOS-reactive neurons in the cortex of cercbral infarcted rats. Methods After 24 h of cerebral infarction rats were divided into different groups randomly; rehabilitation group were given water maze, rotating bar and rolling cage exercises everyday, while immobilization group were fixed in cages. Histochemistry was used to detect the NOS-reactive neurons expression at 1, 2, 3 and 4 week after infarction respectively. Results NOS-reactive neurons existed in normal cortex of both sides. At 24 h after infarction the number increased obviously. And the number of immobilization group decreased more than that of rehabilitation group around the infarction area at 1, 2 week. There was not significantly difference between the two groups at 3, 4 week. Conclusion Rehabilitation training can protect the neurons around the infarction and Thus promote the recovery of rats motor functions.