18F-FECNT的生物分布特性及小动物PET显像研究 |
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引用本文: | 陈正平,王颂佩,李晓敏,刘春仪,唐婕,陆春雄,潘栋辉,徐宇平,杨敏,蒋泉福,黄洪波,周杏琴.18F-FECNT的生物分布特性及小动物PET显像研究[J].中华核医学杂志,2003,29(1):181-184. |
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作者姓名: | 陈正平 王颂佩 李晓敏 刘春仪 唐婕 陆春雄 潘栋辉 徐宇平 杨敏 蒋泉福 黄洪波 周杏琴 |
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作者单位: | 卫生部核医学重点实验室、江苏省原子医学研究所,江苏省无锡市,214063; |
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基金项目: | 国家自然科学基金江苏省高技术研究计划江苏省卫生厅科技项目 |
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摘 要: | Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.
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关 键 词: | 帕金森病 多巴胺 小鼠 大鼠 体层摄影术 发射型计算机 |
Biodistribution and microPET imaging of 18F-FECNT as a dopamine transporter imaging agent |
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Keywords: | FECNTFECNTParkinson diseaseDopamineMiceRatsTomography emission-comouted |
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