| 恶性血液病中的P15^INK4B基因甲基化 |
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| 引用本文： | 吴穷,郭秀枝.恶性血液病中的P15^INK4B基因甲基化[J].中华血液学杂志,2000,21(12):644-646. |
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| 作者姓名： | 吴穷 郭秀枝 |
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| 作者单位： | 暨南大学医学院,广州 |
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| 摘 要： | 目的 探索特定基因操纵区ＣｐＧ岛高甲基化对人类造血系统恶性肿瘤的作用。方法 利用甲基化特异性聚合酶链反应（ＭＳＰ）的方法，用亚硫酸氢钠修饰后的ＤＮＡ为模板进行甲基化特异性聚合酶链反应（ＰＣＲ），测定了２５例急性髓系白血病（ＡＭＬ），１５例慢性粒细胞白血病（ＣＭＬ），１６例骨髓增生异常综合征（ＭＤＳ）和１２例多发性骨髓瘤（ＭＭ）患Ｐ１５＾ＩＮＫ４Ｂ基因在操纵区５′－ＣｐＧ岛异常甲基化的发生率。结果 Ｐ１５＾ＩＮＫ４Ｂ基因异常甲基化的发生率：ＡＭＬ为８４％，ＣＭＬ为０，ＭＤＳ为５０％，ＭＭ为７５％。高危型的ＭＤＳ较低危型更易发生甲基化，在ＭＭ中，Ｐ１５＾ＩＮＫ４Ｂ甲基化一般发生在早期，与骨髓象的幼稚程度关系密切。结论 调节细胞生长和分化的Ｐ１５＾ＩＮＫ４Ｂ基因高甲基化是使Ｐ１５＾ＩＮＫ４Ｂ基因失活的主要原因之一，
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| 关 键 词： | 恶性血液病 P15^INK4B基因 甲基化 基因治疗 |
P15(INK4B) gene methylation in malignant hematopoietic diseases] |
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| Q Wu,X Guo,H Fan,T Zhou,G Tan,Q Guo,P Chen,X Zhang,G Luo,M Xu.P15(INK4B) gene methylation in malignant hematopoietic diseases][J].Chinese Journal of Hematology,2000,21(12):644-646. |
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| Authors: | Q Wu X Guo H Fan T Zhou G Tan Q Guo P Chen X Zhang G Luo M Xu |
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| Institution: | Medical College of Jinan University, Guangzhou 510632, China. |
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| Abstract: | OBJECTIVE: To study the effect of operative region hypermethylation gene in human malignant hematopoietic tumors. METHODS: The abnormal methylation rate of P(15)(INK4B) gene 5'CpG island in 68 cases of malignant hematopoietic tumor samples were determined by methylation specific PCR using bisulfite modified DNA. RESULTS: The methylation rates of P(15)(INK4B) were 84%, 0, 50% and 75%, respectively, for 25 cases of acute myeloid leukemia (AML), 15 chronic myeloid leukemia (CML), 16 myelodysplastic syndrome (MDS) and 12 multiple myeloma (MM). P(15)(INK4B) gene was frequently methylated in patients with high risk MDS and early stage of MM. CONCLUSION: Hypermethylation of P(15)(INK4B) gene is one of the main causes of its inactivation. Hypermethylation of CpG island was closely related to the development of malignant hematopoietic diseases. |
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