| 人质膜型唾液酸酶与人红白血病细胞多药耐药相关 |
| |
| 引用本文： | 许莉,孙连坤,李峰,禹彬,李晓洁,赵雪俭,李扬.人质膜型唾液酸酶与人红白血病细胞多药耐药相关[J].基础医学与临床,2010,30(2):139-143. |
| |
| 作者姓名： | 许莉 孙连坤 李峰 禹彬 李晓洁 赵雪俭 李扬 |
| |
| 作者单位： | 1. 吉林大学白求恩医学院病理生理教研室,吉林 长春 130021;东华大学化学化工与生物工程学院,上海 201620
2. 吉林大学白求恩医学院病理生理教研室,吉林 长春,130021 |
| |
| 基金项目： | 吉林省科学技术发展项目(20070728-1) |
| |
| 摘 要： | 目的探讨人质膜型唾液酸酶(Neu3)与人红白血病细胞多药耐药的关系。方法分别或联合柔红霉素(DNR)与唾液酸酶特异性抑制剂(NeuAC2en)处理K562和K562/ADM细胞,MTT法检测细胞生存率;RT-PCR检测多药耐药基因(MDR)、多药耐药相关蛋白(MRP)、06-甲基鸟嘌呤-DNA甲基转移酶(MGMT)、谷胱甘肽S转移酶(GST)、BCL-xl、BAX和BCL-2mRNA表达;Western blot法检测MDR基因蛋白产物P-糖蛋白(P-gp)蛋白表达;TBA法检测唾液酸酶活性。结果与K562细胞相比,K562/ADM细胞对DNR具有一定的抵抗性,NeuAC2en与DNR有协同作用(P<0.01);应用NeuAC2en或DNR后K562和K562/ADM细胞Neu3活性均明显下降,两种药物联合应用Neu3活性下降最明显(P<0.01);相同处理条件下,与K562细胞相比,K562/ADM细胞中Neu3、MDR1、BCL-xl和BCL-2表达增加,BAX无明显变化;应用DNR后,MDR1、Neu3、BCL-xl、BCL-2表达均下降,而DNR与NeuAC2en联合应用,以上基因表达水平下调最明显。...
|
| 关 键 词： | 人质膜型唾液酸酶 多药耐药 K562 |
| 收稿时间： | 2009-2-13 |
| 修稿时间： | 2009-8-24 |
Relationship between human membrane associated sialidase ( Neu3 ) and multidrug resistance (MDR) in K562 cell line |
| |
| XU Li,SUN Lian-kun,LI Feng,YU Bin,LI Xiao-jie,ZHAO Xue-jian,LI Yang.Relationship between human membrane associated sialidase ( Neu3 ) and multidrug resistance (MDR) in K562 cell line[J].Basic Medical Sciences and Clinics,2010,30(2):139-143. |
| |
| Authors: | XU Li SUN Lian-kun LI Feng YU Bin LI Xiao-jie ZHAO Xue-jian LI Yang |
| |
| Institution: | 1.Dept.of Pathophysiology;Norman Bathune College of Medical Sciences;Jilin University;Changchun 130021;2.College of Chemistry;Chemical Engineering and Biotechnology;Donghua University;Shanghai 201620;China |
| |
| Abstract: | Objective To investigate the role of human membrane associated sialidase(Neu3) in multidrug resistance(MDR) of K562 cells.Methods K562 cells and K562/ADM cells were treated with DNR alone or with combination of DNR and NeuAC2en.The cell survival rate was measured by MTT assay;the expression of MDR related factors and apoptosis related factors were determined by Western blot and RT-PCR;Neu3 activity was detected by TBA reaction.Results The survival rate of K562/ADM cells was higher than that of K562 cells;Ne... |
| |
| Keywords: | human membrane associated sialidase multidrug resistance K562 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《基础医学与临床》浏览原始摘要信息 |
| 点击此处可从《基础医学与临床》下载免费的PDF全文 |
|
