碱性成纤维细胞生长因子是预测肝细胞癌根治性切除术后转移复发的潜在指标

目的 检测肝细胞癌（hepatocellular carcinoma,HCC)患者术前血清碱性成纤维细胞生长因子（basic fibroblast growth,bFGF)水平，确定其与HCC根治性术后转移复发的关系。方法 采用Cox比例风险模型，对影响预后的临床指标进行分析，确定影响HCC切除术后转移复发的临床指标。同时应用ELISA方法，检测HCC患者术前血清bFGF水平，western blot方法对24例相应肿瘤组织bFGF表达水平进行定量分析。结果 Cox比例风险模型显示，肿瘤大小及肿瘤是否存在肝内播散，是影响HCC切除术后患者生存时间的显著因素（分别为P＝0．022和P＝0．040），并以此划分为高和传转移复发倾向组。根治性切除术后高转移复发倾向组，HCC患者术前血清bFGF显著高于传转移复发倾向组（P＜0．01）。western blot显示，HCC患者血清bFGF水平变化与其对应肿瘤组织bFGF表达变化相一致。结论 HCC患者术前血清bFGF是预测HCC根治性切除术后转移复发的潜在指标。HCC患者血清bFGF水平升高是其对应肿瘤组织高表达的结果。

Serum bFGF is a potential predictor for metastatic recurrence after curative resection of hepatocellular carcinoma

Objective To study the significance of serum basic fibroblast growth factor (bFGF) as a predictor for metastatic recurrence after curative resection of hepatocellular carcinoma (HCC). Method Cox's proportional hazard model was used to determine the significant clinical factors of prognosis. Meanwhile, enzyme linked immunosorbent assay (ELISA) and Western Blot analysis were used to test serum and tumor tissue levels of bFGF respectively in HCC patients. Results Cox's proportional hazard model showed that tumor size and tumor number were significant prognostic factors of HCC patients after hepatic resection ( P =0.022 and P =0.040, respectively). Based on this results, HCC patients in our studies were divided into high tendency to metastatic recurrence and low tendency to metastatic recurrence groups. Serum levels of bFGF in high tendency to metastatic recurrence group were significantly higher than those in low tendency to metastatic recurrence group ( P <0.01). Western Blot analysis revealed that changes of bFGF level in the serum were consistent with those in the tumor tissues of HCC patients. Conclusion Serum bFGF is a potential predictor of metastatic recurrence after curative resection of HCC. The higher levels of bFGF in the serum are the results of higher bFGF expressions in the tumor tissues of HCC patients.