冻干牛血中硒成分分析标准物质的研制

牛血样品通过低温冷冻、解冻及离心处理,除去细胞碎片后分成2份,1份为低水平,另1份加入Se~(+4)(高水平)。2份样品分别以2ml/瓶的量分装在棕色瓶中,冷冻干燥,辐射灭菌,最后于0℃以下保存。用荧光分光光度法测其均匀性与稳定性。结果本标准物质至少可以稳定1年。采用多个实验室多种方法确定的标准值为:低水平:(99.9±6.9)μg/L,高水平:(981±42)μg/L。

Development of the certified reference material of selenium in lyophilized bovine blood

Samples of bovine blood were frosted, defrosted and centrifuged to destroy and separate blood cells. The blood was processed in two lots: the first lot was of low level, the second lot was fortified with Se~(+4) (high level) and homogenized. The two lots of blood samples were dispensed into brown glass bottles (2.0 ml per bottle), separately, and then lyophilized, radio-sterilized, and stored at less than 0℃. Homogeneity test, stability inspection and certification were conducted using a fluorescence spectrophotometric method. The physical and chemical stability of the CRM was assessed for one year. The certified values are based on analysis made by a group of independent laboratoriees using fluorescence spectrophotometric method, atomic absorption spectrophotometric method and atomic fluorescence spectrophotomdtric method. The certified values±uncertainties are as follows: Low level: 9.0±6.9μg/L, Elevated level: 981±42μg/L.