| 石蜡包埋的石棉肺癌组织的基因组DNA的快速提取法 |
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| 引用本文： | 付德辰,刘秉慈.石蜡包埋的石棉肺癌组织的基因组DNA的快速提取法[J].卫生研究,1996,25(4):204-205. |
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| 作者姓名： | 付德辰 刘秉慈 |
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| 作者单位： | 中国预防医学科学院劳动卫生与职业病研究所 |
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| 基金项目： | 本课题为中国预防医学科学院科研基金资助项目 |
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| 摘 要： | 建立了从少量石蜡包埋的组织切片中快速提取石棉肺癌组织中的基因组ＤＮＡ的水煮脱蜡法，克服了传统的二甲苯脱蜡法的周期长、成本高和毒性大的缺点。所得到的基因组ＤＮＡ中，长片段占较大的比例，总量可足够用作１０～２０次ＰＣＲ扩增反应的模板，并可用于其它分子生物学研究
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| 关 键 词： | 石蜡包埋 石棉肺癌 基因组DNA提取 |
Rapid extraction of genomic DNA from paraffin embedded asbestos related lung cancer tissues |
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| Fu Dechen, Liu Bingci, Miao Qing, Wang Haihua,et al..Rapid extraction of genomic DNA from paraffin embedded asbestos related lung cancer tissues[J].Journal of Hygiene Research,1996,25(4):204-205. |
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| Authors: | Fu Dechen Liu Bingci Miao Qing Wang Haihua |
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| Abstract: | rapid method of extraction of genomic DNA from small amount of paraffin embedded tissues was developed in this experiment. This method overcame the deficiency of the classical method, which is time consuming and with severe toxicity. The genomic DNA prepared in this method had many long fragments and they could be used to prepare temples for PCR or be used in other molecular biology research. |
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| Keywords: | paraffin embedded asbestos related lung cancer genomic DNA extraction |
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