硒对镉在大鼠肝脏亚细胞分布及脂质过氧化作用的影响

采用给大鼠腹腔注射镉(CdCl_2 1mg/kg)或/和硒(Na_2SeO_3 0.4mg/kg)的方法,研究了硒对镉在大鼠肝脏亚细胞分布及脂质过氧化作用的影响。结果表明,给大鼠连续腹腔注射CdCl_2(1mg/kg)3d,可致大鼠肝微粒体和线粒体脂质过氧化物(LPO)含量显著增加,谷胱甘肽过氧化物酶(GSH-Px)活性显著下降,肝细胞浆还原型谷胱甘肽(GSH)含量显著降低。同时给大鼠腹腔注射Na_2SeO_3(0.4mg/kg),硒可拮抗上述镉的效应。但硒对大鼠肝脏镉含量及镉在肝脏亚细胞分布没有明显影响。

The effect of selenium on subcellular distribution of cadmium and lipid peroxidation in rat liver

The effect of selenium on subcellular distribution of cadmium and lipid peroxidation in rat liver was studied by administering ip. Na_2SeO_3(0.4mg/kg) or/and CdCl_2 (1mg/kg) for 3 days. The results showed that the content of lipid peroxide (LPO) increased, the activity of glutathione peroxidase (GSH-Px) decreased siginficantly in rat liver microsome and mitchondria, and the content of glutathione(GSH) in rat liver cytosol decreased obviously after injection of CdCl_2 for 3 days alone. The lipid peroxidation of rat liver caused by CdCl_2 was antagonized by the injection of Na_2SeO_3 simultaneously, but the content of liver cadmium and subcellular distribution of cadmium was not affected by the injection of Na_2SeO_3.