| 壁虎骨骼肌卫星细胞的原代培养与鉴定 |
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| 引用本文： | 徐青,徐敏,张加吉,李冬辉,刘炎.壁虎骨骼肌卫星细胞的原代培养与鉴定[J].中国交通医学杂志,2011,25(2):110-112,117. |
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| 作者姓名： | 徐青 徐敏 张加吉 李冬辉 刘炎 |
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| 作者单位： | 徐青,张加吉,李冬辉,刘炎,XU Qing,ZHANG Jiaji,LI Donghui,LIU Yan(南通大学神经再生重点实验室);徐敏,XU Min(南通大学医学院形态学实验室,江苏,226001) |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:建立一种有效的壁虎骨骼肌卫星细胞分离、培养及鉴定体系。方法:取壁虎后肢肌,采用I型胶原酶消化法获得细胞悬液,经差速贴壁法纯化骨骼肌卫星细胞,通过Pax7免疫细胞化学方法鉴定骨骼肌卫星细胞。用此法也分离到小鼠骨骼肌卫星细胞。结果:(1)分离到的细胞呈小圆形,培养2天后可见纺锤形细胞。(2)免疫细胞化学染色显示,分离得到的壁虎骨骼肌卫星细胞表达Pax7。Pax7阳性的小鼠骨骼肌卫星细胞达99%以上。培养3天后卫星细胞开始增殖。结论:成功建立壁虎骨骼肌卫星细胞的纯化和鉴定方法,为进一步研究卫星细胞在高等哺乳动物损伤修复中的可塑性奠定基础。
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| 关 键 词： | 骨骼肌卫星细胞 原代培养 差速贴壁法 壁虎 |
Primary cultivation and identification of satellite cells of Gekko japonicus skeletal muscles |
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| XU Qing,XU Min,ZHANG Jiaji,LI Donghui,LIU Yan.Primary cultivation and identification of satellite cells of Gekko japonicus skeletal muscles[J].Chinese Medical JOurnal of Communications,2011,25(2):110-112,117. |
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| Authors: | XU Qing XU Min ZHANG Jiaji LI Donghui LIU Yan |
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| Institution: | XU Qing1,XU Min2,ZHANG Jiaji1,LI Donghui1,LIU Yan1(1Key Laboratory of Neuroregeneration,Nantong University,2Medical School of Nantong University,Jiangsu 226001) |
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| Abstract: | Objective: To establish a method for isolation,culture and identification of satellite cells from gecko skeletal muscles.Methods: Cell suspension was obtained from posterior limb skeletal muscles of gecko by collagenase I digestion,and the muscle satellite cells were purified by the method of differential adherence.Finally satellite cells were identified with monoclonal antibody against Pax7.Satellite cells of mice skeletal muscles were obtained in accordance with this method as well.Results:(1)The cells is... |
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| Keywords: | skeletal muscle satellite cell differential adherence gecko |
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