| 外源人核受体hLRH-1在结肠腺癌细胞SW480中表达的生物学效应及其分子机理初探 |
| |
| 引用本文： | Shuiliang Wang,Yingmeng Zheng,Fenghua Lan.外源人核受体hLRH-1在结肠腺癌细胞SW480中表达的生物学效应及其分子机理初探[J].中德临床肿瘤学杂志,2007,6(3):P228-P231. |
| |
| 作者姓名： | Shuiliang Wang Yingmeng Zheng Fenghua Lan |
| |
| 作者单位： | [1]Center for Molecular Medicine, Institute of Laboratory Medicine, Fuzhou General Hospital, Fuzhou 350025, China [2]Department of Laboratory Medicine, First Hospital of Q.uanzhou, Q.uanzhou 362000, China |
| |
| 基金项目： | 福建省青年科技人才创新基金
,
福州总医院博士基金 |
| |
| 摘 要： | Objective： To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer. Methods： Plasmids pcDNA3-hLRH-1 were introduced into SW480 cells via lipofectamine. The expression of mRNA and protein of exogenous hLRH-1 were detected by RT-PCR and western blotting, respectively. MTT assay was carried out to survey the proliferation of SW480 cells with overexpression of hLRH-1. Meanwhile, the expression of proliferation-related genes cyclin E1 and cyclin D1, and apoptosis-related genes PTEN and Rbl, were analyzed by realtime RT-PCR. Results： The proliferation of SW480 cells was promoted under the condition of overexpression of hLRH-1. The expression of cyclin E1 was up-regulated significantly, while that of PTEN and Rbl were down-regulated in SW480 cells with overexpressed hLRH-1. Conclusion： The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin E1, as well as participated in the regulation of apoptosis via influencing the expression of PTEN and Rb1.
|
| 关 键 词： | 生物治疗 克隆技术 癌细胞 核磁疗法 |
| 收稿时间： | 2 February 2007 |
| 修稿时间： | 2007-02-022007-05-05 |
Biological effect of expression of exogenous human nuclear receptor hLRH-1 in SW480 cells and its molecular mechanism |
| |
| Shuiliang?Wang,Yingmeng?Zheng,Fenghua?Lan.Biological effect of expression of exogenous human nuclear receptor hLRH-1 in SW480 cells and its molecular mechanism[J].The Chinese-German Journal of Clinical Oncology,2007,6(3):P228-P231. |
| |
| Authors: | Shuiliang Wang Yingmeng Zheng Fenghua Lan |
| |
| Institution: | (1) Center for Molecular Medicine, Institute of Laboratory Medicine, Fuzhou General Hospital, Fuzhou, 350025, China;(2) Department of Laboratory Medicine, First Hospital of Quanzhou, Quanzhou, 362000, China |
| |
| Abstract: | Objective:To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer.Methods:Plasmids pcDNA3-hLRH-1 were introduced into SW480 ceils via lipofectamine.The expression of mRNA and protein of exogenous hLRH-1 were detected by RT-PCR and western blotting.respectively.MTT assay was carried out to survey the proliferation of SW80 cells with overexpression of hLRH-1.Meanwhile.the expression of proliferation-related genes cyclin E1 and cyclin D1.and apoptosis-related genes PTEN and Rb1.were analyzed by realtime RT-PCR.Result:The proliferation of SW480 cells was promoted under the condition of overexpression of hLRH-1.The expression of cyclin E1 was up-regulated significantly,while that of PTEN and Rb1 were down-regulated jn SW480 cells with overexpressed hLRH-1.Conclusion:The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin E1.as well as participated in the regulation of apoptosis via influencing the expression of PTEN and Rb1. |
| |
| Keywords: | nuclear receptor hLRH-1:colon cancer expression of exogenous gene biological effect |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
